Oxygen Generating Polymeric Nano Fibers That Stimulate Angiogenesis and Show Efficient Wound Healing in a Diabetic Wound Model.
Animals
Bandages
Biological Assay
Chorioallantoic Membrane
/ drug effects
Diabetes Mellitus
/ pathology
Diabetes Mellitus, Experimental
/ pathology
Disease Models, Animal
Gene Expression Regulation
/ drug effects
Humans
Male
Nanofibers
/ chemistry
Neovascularization, Physiologic
/ drug effects
Oxygen
/ chemistry
Polyesters
/ pharmacology
Rats, Sprague-Dawley
Reproducibility of Results
Skin
/ pathology
Spectroscopy, Fourier Transform Infrared
Wound Healing
/ drug effects
diabetic wound healing
electrospinning
oxygenation
polymeric nanofibers
skin regeneration
Journal
International journal of nanomedicine
ISSN: 1178-2013
Titre abrégé: Int J Nanomedicine
Pays: New Zealand
ID NLM: 101263847
Informations de publication
Date de publication:
2020
2020
Historique:
received:
08
02
2020
accepted:
29
04
2020
entrez:
18
6
2020
pubmed:
18
6
2020
medline:
6
8
2020
Statut:
epublish
Résumé
Diabetic wounds are challenging to treat due to a wide range of pathophysiological changes. Hypoxia is one of the predominant contributing factors of poor vascularization and chronicity in diabetic wounds. This study was designed to develop polycaprolactone (PCL)-based oxygen-releasing electrospun wound dressings and evaluate their efficacy for improved full thickness wound healing in diabetic rats. PCL-based oxygen releasing wound dressings were made using electrospinning technology. The developed dressings were characterized in terms of physical as well as biological properties both in vitro and in vivo. E-spun nanofibrous dressings were physically characterized with scanning electron microscopy, Fourier-transform infrared spectroscopy, and Energy-dispersive X-ray spectroscopy. To study the likely impact of the fabricated wound dressings in hypoxic conditions, HIF-1α expression analysis was carried out both at gene and protein levels. Wound dressings were further evaluated for their healing potential for extensive wounds in diabetic rat models. The experimental results showed that the developed dressings were capable of continuously generating oxygen for up to 10 days. Cell studies further confirmed pronounced expression of HIF-1α at gene and protein levels in cells seeded on PCL-sodium percarbonate (SPC) and PCL scaffolds compared with the cells cultured on a tissue culture plate. Chorioallantoic membrane assay revealed the supportive role of oxygen releasing dressings on angiogenesis compared to the control group. Histological assessment of the regenerated skin tissues proved that full thickness wounds covered with SPC loaded PCL dressings had a comparatively better vascularized and compact extracellular matrix with completely covered thick epithelium. The developed oxygen generating polymeric nanofibrous wound dressings could potentially be used as an envisioned approach for the efficient recovery of chronic diabetic wounds.
Identifiants
pubmed: 32547010
doi: 10.2147/IJN.S248911
pii: 248911
pmc: PMC7244241
doi:
Substances chimiques
Polyesters
0
polycaprolactone
24980-41-4
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3511-3522Informations de copyright
© 2020 Zehra et al.
Déclaration de conflit d'intérêts
The authors report no conflicts of interest in this work.
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