Gastrointestinal Tract Colonization Rate of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae and Associated Factors Among Hospitalized Patients in Arba Minch General Hospital, Arba Minch, Ethiopia.
ESBL
colonization
enterobacteriaceae
gastrointestinal tract
susceptibility
Journal
Infection and drug resistance
ISSN: 1178-6973
Titre abrégé: Infect Drug Resist
Pays: New Zealand
ID NLM: 101550216
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
11
2019
accepted:
07
05
2020
entrez:
18
6
2020
pubmed:
18
6
2020
medline:
18
6
2020
Statut:
epublish
Résumé
The incidence of hospital-acquired enterobacteria that produce extended-spectrum beta-lactamases (ESBLs) is on the rise worldwide. Colonization of gastrointestinal tract by extended-spectrum beta-lactamase Enterobacteriaceae, a prominent causative agent, results in life-threatening infections. To determine the rate of gastrointestinal colonization by extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae and also to elucidate the antibiotic susceptibility profile and associated risk factors among hospitalized patients in Arba Minch General Hospital, Ethiopia. A facility-based cross-sectional study was conducted in Arba Minch General Hospital from May 2018 to July 2019. Sociodemographic data and associated factors were collected using a pre-tested-structured questionnaire. Stool specimens were collected using sterile stool cups. Each sample was then inoculated onto MacConkey agar. Bacterial isolates were identified using various biochemical tests. Screening and confirmatory tests for extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae were performed using the modified Kirby-Bauer disc diffusion technique. Statistical package for Social Science was used to analyze the data. The P-value ≤0.05 was considered as statistically significant. A total of 421 hospitalized patients were enrolled in this study of which there were 240 (57%) females. The mean age of the study participants was 28.8 with SD of 15.7. Majority of participants were in the age range of 25-40 years 179 (42.5%). About 146 (34.7%) participants were found to be colonized by extended-spectrum beta-lactamase-producing Enterobacteriaceae. The predominant ESBL-producing isolates were The overall colonization rate of the gastrointestinal tract by extended-spectrum beta-lactamase-producing Enterobacteriaceae was prominent. The extended-spectrum beta-lactamase-producing isolates exhibited a higher level of resistance against the commonly used antibiotics which further needs greater attention.
Sections du résumé
BACKGROUND
BACKGROUND
The incidence of hospital-acquired enterobacteria that produce extended-spectrum beta-lactamases (ESBLs) is on the rise worldwide. Colonization of gastrointestinal tract by extended-spectrum beta-lactamase Enterobacteriaceae, a prominent causative agent, results in life-threatening infections.
OBJECTIVE
OBJECTIVE
To determine the rate of gastrointestinal colonization by extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae and also to elucidate the antibiotic susceptibility profile and associated risk factors among hospitalized patients in Arba Minch General Hospital, Ethiopia.
METHODOLOGY
METHODS
A facility-based cross-sectional study was conducted in Arba Minch General Hospital from May 2018 to July 2019. Sociodemographic data and associated factors were collected using a pre-tested-structured questionnaire. Stool specimens were collected using sterile stool cups. Each sample was then inoculated onto MacConkey agar. Bacterial isolates were identified using various biochemical tests. Screening and confirmatory tests for extended-spectrum beta-lactamase- and carbapenemase-producing Enterobacteriaceae were performed using the modified Kirby-Bauer disc diffusion technique. Statistical package for Social Science was used to analyze the data. The P-value ≤0.05 was considered as statistically significant.
RESULTS
RESULTS
A total of 421 hospitalized patients were enrolled in this study of which there were 240 (57%) females. The mean age of the study participants was 28.8 with SD of 15.7. Majority of participants were in the age range of 25-40 years 179 (42.5%). About 146 (34.7%) participants were found to be colonized by extended-spectrum beta-lactamase-producing Enterobacteriaceae. The predominant ESBL-producing isolates were
CONCLUSION
CONCLUSIONS
The overall colonization rate of the gastrointestinal tract by extended-spectrum beta-lactamase-producing Enterobacteriaceae was prominent. The extended-spectrum beta-lactamase-producing isolates exhibited a higher level of resistance against the commonly used antibiotics which further needs greater attention.
Identifiants
pubmed: 32547121
doi: 10.2147/IDR.S239092
pii: 239092
pmc: PMC7250175
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1517-1526Informations de copyright
© 2020 Aklilu et al.
Déclaration de conflit d'intérêts
The authors declared that they have no conflicts of interest in this work.
Références
Infect Control Hosp Epidemiol. 2017 May;38(5):580-594
pubmed: 28294079
Infect Control Hosp Epidemiol. 2009 Jun;30(6):534-42
pubmed: 19419270
J Infect Dev Ctries. 2013 Aug 15;7(8):630-4
pubmed: 23949299
Clin Microbiol Infect. 2012 Mar;18(3):268-81
pubmed: 21793988
J Travel Med. 2017 Apr 1;24(suppl_1):S29-S34
pubmed: 28520999
Infect Dis (Auckl). 2014 Mar 25;7:1-8
pubmed: 24847178
BMC Infect Dis. 2017 Mar 29;17(1):237
pubmed: 28356079
PLoS One. 2016 Dec 9;11(12):e0168024
pubmed: 27936054
Pediatr Infect Dis J. 2016 Aug;35(8):856-61
pubmed: 27124686
Drugs. 2003;63(4):353-65
pubmed: 12558458
J Med Microbiol. 2011 May;60(Pt 5):619-624
pubmed: 21292857
PLoS One. 2016 Aug 30;11(8):e0161685
pubmed: 27574974
Turk J Med Sci. 2016 Feb 27;47(1):172-179
pubmed: 28263486
Antimicrob Resist Infect Control. 2017 Jun 13;6:62
pubmed: 28630686
J Glob Antimicrob Resist. 2017 Mar;8:90-96
pubmed: 28039104
Emerg Infect Dis. 2007 Aug;13(8):1144-9
pubmed: 17953083
J Antimicrob Chemother. 2010 Jul;65(7):1545-6
pubmed: 20483982
Clin Microbiol Rev. 2013 Apr;26(2):289-307
pubmed: 23554418
Infect Ecol Epidemiol. 2014 Oct 01;4:
pubmed: 25317262
Infect Control Hosp Epidemiol. 2012 Dec;33(12):1242-5
pubmed: 23143363
PLoS One. 2013 Oct 11;8(10):e76597
pubmed: 24146896