S1P promotes IL-6 expression in osteoblasts through the PI3K, MEK/ERK and NF-κB signaling pathways.
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Humans
Interleukin-6
/ metabolism
Lysophospholipids
/ pharmacology
NF-kappa B
/ metabolism
Osteoblasts
/ drug effects
Phosphatidylinositol 3-Kinases
/ metabolism
Real-Time Polymerase Chain Reaction
Signal Transduction
/ drug effects
Sphingosine
/ analogs & derivatives
Synovial Fluid
/ metabolism
Arthritis
IL-6
Osteoblasts
S1P
Journal
International journal of medical sciences
ISSN: 1449-1907
Titre abrégé: Int J Med Sci
Pays: Australia
ID NLM: 101213954
Informations de publication
Date de publication:
2020
2020
Historique:
received:
06
02
2020
accepted:
27
04
2020
entrez:
18
6
2020
pubmed:
18
6
2020
medline:
9
3
2021
Statut:
epublish
Résumé
Rheumatoid arthritis (RA) is a systemic autoimmune inflammatory disease, in which the immune system attacks joint tissue. Interleukin (IL)-6 is a key proinflammatory cytokine in RA progression. Sphingosine-1-phosphate (S1P), a platelet-derived lysophospholipid mediator, reportedly regulates osteoimmunology. Here, we examined the effects of S1P on IL-6 expression in osteoblasts. Our results and records from the Gene Expression Omnibus (GEO) database demonstrate higher levels of IL-6 in patients with RA compared with those with osteoarthritis. Stimulation of osteoblasts with S1P increased mRNA and protein expression of IL-6. PI3K, MEK, ERK and NF-κB inhibitors and their small interfering RNAs (siRNAs) reduced S1P-promoted IL-6 expression. S1P also facilitated PI3K, MEK/ERK and NF-κB signaling cascades. Our results indicate that S1P promotes the expression of IL-6 in osteoblasts via the PI3K, MEK/ERK and NF-κB signaling pathways.
Identifiants
pubmed: 32547316
doi: 10.7150/ijms.44612
pii: ijmsv17p1207
pmc: PMC7294913
doi:
Substances chimiques
Interleukin-6
0
Lysophospholipids
0
NF-kappa B
0
sphingosine 1-phosphate
26993-30-6
Sphingosine
NGZ37HRE42
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1207-1214Informations de copyright
© The author(s).
Déclaration de conflit d'intérêts
Competing Interests: The authors have declared that no competing interest exists.
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