Selective Visualization of Caveolae by TEM Using APEX2.

Animals Caveolae / metabolism Caveolin 1 / metabolism Cell Line DNA-(Apurinic or Apyrimidinic Site) Lyase / chemistry Endonucleases / chemistry Humans Microscopy, Electron, Transmission / methods Multifunctional Enzymes / chemistry RNA-Binding Proteins / metabolism Tomography / methods
3D tomography APEX2 Caveolae Caveolin Cavin Correlative light and electron microscopy TEM

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 18 6 2020
pubmed: 18 6 2020
medline: 1 4 2021
Statut: ppublish

Résumé

Caveolae are small flask- or cup-shaped invaginations of the plasma membrane found in almost all vertebrate cells. Due to their small size (50-100 nm), transmission electron microscopy (TEM) has been the method of choice to study caveolae formation and ultrastructure and, more recently, to resolve the sub-caveolar localization of its protein components using novel protein labeling methods for TEM. This chapter describes a protocol for the selective visualization of caveolae and caveolar proteins by TEM, 3D tomography, and correlative light and electron microscopy (CLEM) using the peroxidase APEX2.

Identifiants

DOI: 10.1007/978-1-0716-0732-9_1 PMID: 32548814
pubmed: 32548814
doi: 10.1007/978-1-0716-0732-9_1
doi:

Substances chimiques

CAVIN1 protein, human 0
Caveolin 1 0
Multifunctional Enzymes 0
RNA-Binding Proteins 0
Endonucleases EC 3.1.-
APEX2 protein, human EC 4.2.99.18
DNA-(Apurinic or Apyrimidinic Site) Lyase EC 4.2.99.18

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1-10

Auteurs

Alexander Ludwig (A)

School of Biological Sciences, NTU Institute of Structural Biology, Nanyang Technological University, Singapore, Singapore. ALudwig@ntu.edu.sg.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Vésicules de transport › Vésicules tapissées › Cavéoles › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Cavéoline-1 › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-oxygen lyases › DNA-(apurinic or apyrimidinic site) lyase › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Endonucleases › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Enzymes multifonctionnelles › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Selective Visualization of Caveolae by TEM Using APEX2.
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Tomographie › Selective Visualization of Caveolae by TEM Using APEX2.