A detached leaf assay for testing transient gene expression and gene editing in cowpea (
CRISPR/Cas9
Cowpea
Genome editing
Leaf
Meiosis
Plant reproduction
Transient assay
Journal
Plant methods
ISSN: 1746-4811
Titre abrégé: Plant Methods
Pays: England
ID NLM: 101245798
Informations de publication
Date de publication:
2020
2020
Historique:
received:
14
04
2020
accepted:
06
06
2020
entrez:
19
6
2020
pubmed:
19
6
2020
medline:
19
6
2020
Statut:
epublish
Résumé
The legume cowpea ( Attempts to develop a transient protoplast system for cowpea were unsuccessful. Leaflets from plants 3-4 weeks post-germination were age selected to establish a rapid The transient, detached cowpea leaf assay, and supporting analytical methods developed, provide a rapid and reproducible means for testing gene expression constructs, and constructs for inducing mutagenesis in genes involved in both vegetative and reproductive developmental programs. The method and tested editing constructs and components have potential application for a range of crop legumes.
Sections du résumé
BACKGROUND
BACKGROUND
The legume cowpea (
RESULTS
RESULTS
Attempts to develop a transient protoplast system for cowpea were unsuccessful. Leaflets from plants 3-4 weeks post-germination were age selected to establish a rapid
CONCLUSION
CONCLUSIONS
The transient, detached cowpea leaf assay, and supporting analytical methods developed, provide a rapid and reproducible means for testing gene expression constructs, and constructs for inducing mutagenesis in genes involved in both vegetative and reproductive developmental programs. The method and tested editing constructs and components have potential application for a range of crop legumes.
Identifiants
pubmed: 32549904
doi: 10.1186/s13007-020-00630-4
pii: 630
pmc: PMC7296760
doi:
Types de publication
Journal Article
Langues
eng
Pagination
88Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing interestsNo potential conflicts of interest were disclosed.
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