Effects of erythropoietin on bacterial translocation in a rat model of experimental colitis.
Erythropoietin
bacterial translocation
experimental colitis
Journal
Turkish journal of surgery
ISSN: 2564-6850
Titre abrégé: Turk J Surg
Pays: Turkey
ID NLM: 101704837
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
30
08
2018
accepted:
08
10
2018
entrez:
19
6
2020
pubmed:
19
6
2020
medline:
19
6
2020
Statut:
epublish
Résumé
In this experimental study, it was aimed to assess the effects of erythropoietin (EPO) on bacterial translocation in a rat model of colitis. The rats were randomly assigned into control, colitis and EPO-treated groups (n= 8 in each group). Saline solution (NS) was administered to control rats via rectal route. A trinitrobenzene sulfonic acid and ethanol mixture (TNBS-E) was used to induce colitis in the experiment groups. No treatment was administered to colitis group after induction. Starting at one day after induction of colitis with TNBS-E, EPO (1000 IU/kg) was administered subcutaneously for three days to the rats in the EPO-treated group. Colonic inflammation was assessed by gross and microscopic examination on day five. Blood samples were obtained to evaluate bacterial translocation while hepatic, mesenteric tissue samples and mesenteric lymph node (MLN) samples were collected for tissue culture. Tissue myeloperoxidase (MPO) levels, and tumor necrosis factor alpha (TNF- α) and endotoxin levels in the sera were studied. Significant gross and microscopic differences were found in the comparison between colitis and EPO-treated groups (p <0.05). MPO level was significantly lower when compared to the colitis group (p <0.05). Serum TNF-α and plasma endotoxin levels were significantly lower in the EPO-treated group than the colitis group (p <0.05). Bacterial translocation was lower in the liver, spleen, MLNs and systemic blood in the EPO-treated group when compared to the colitis group (p <0.05). In TNBS-E-induced rat model of colitis, EPO significantly decreased inflammation and bacterial translocation based on histopathological, biochemical and microbiological parameters.
Identifiants
pubmed: 32550329
doi: 10.5578/turkjsurg.4272
pmc: PMC6795224
doi:
Types de publication
Journal Article
Langues
eng
Pagination
202-209Informations de copyright
Copyright © 2019, Turkish Surgical Society.
Déclaration de conflit d'intérêts
Conflict of Interest: No conflict of interest was declared by the authors.
Références
Gastroenterology. 1984 Dec;87(6):1344-50
pubmed: 6092199
Res Pharm Sci. 2012 Jul;7(3):159-69
pubmed: 23181094
J Immunol. 2000 May 1;164(9):4878-82
pubmed: 10779797
Mediators Inflamm. 2016;2016:1346390
pubmed: 27990061
Acta Med Iran. 2016 Oct;54(10):624-630
pubmed: 27888589
Gastroenterology. 1989 Jan;96(1):29-36
pubmed: 2535830
Gut. 1998 Jan;42(1):29-35
pubmed: 9505882
Science. 2005 Mar 25;307(5717):1915-20
pubmed: 15790844
Brain Res. 2002 Oct 11;952(1):128-34
pubmed: 12363412
Basic Clin Pharmacol Toxicol. 2017 Feb;120(2):138-145
pubmed: 27579991
Fundam Clin Pharmacol. 2013 Jun;27(3):299-307
pubmed: 22151426
N Engl J Med. 2002 Aug 8;347(6):417-29
pubmed: 12167685
Gut. 2003 Sep;52(9):1291-6
pubmed: 12912860
Turk J Gastroenterol. 2012;23(6):691-8
pubmed: 23794307
Gut. 1991 Aug;32(8):913-7
pubmed: 1885073
Rev Hosp Clin Fac Med Sao Paulo. 1997 Jul-Aug;52(4):180-6
pubmed: 9567368
J Gastroenterol Hepatol. 2007 Apr;22(4):464-71
pubmed: 17376034
J Pharmacol Exp Ther. 2004 Dec;311(3):1272-80
pubmed: 15302898
World J Gastroenterol. 2009 May 7;15(17):2067-73
pubmed: 19418577
Am J Surg. 2006 Jan;191(1):77-83
pubmed: 16399111
J Trauma. 2011 Nov;71(5 Suppl 1):S456-61
pubmed: 22072003
Gastroenterol Clin North Am. 2002 Mar;31(1):1-20
pubmed: 12122726
J Pharmacol Toxicol Methods. 2004 Sep-Oct;50(2):81-92
pubmed: 15385082
Dig Dis Sci. 2009 Mar;54(3):471-80
pubmed: 18618255
Neural Regen Res. 2016 Sep;11(9):1450-1455
pubmed: 27857749
Surgery. 2008 Apr;143(4):556-65
pubmed: 18374054
PLoS One. 2013;8(1):e54543
pubmed: 23382912
Hematol Rep. 2014 Dec 04;6(4):5600
pubmed: 25568760
Immunity. 2011 Jan 28;34(1):61-74
pubmed: 21256055
Nat Rev Immunol. 2003 Jul;3(7):521-33
pubmed: 12876555
Ann Ital Chir. 2014 Mar-Apr;85(2):159-65
pubmed: 24901383
Ann Intern Med. 1993 Oct 15;119(8):771-8
pubmed: 8379598