Protective effects of spironolactone against hepatic ischemia/reperfusion injury in rats.
Journal
Turkish journal of surgery
ISSN: 2564-6850
Titre abrégé: Turk J Surg
Pays: Turkey
ID NLM: 101704837
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
25
10
2018
accepted:
04
01
2019
entrez:
20
6
2020
pubmed:
20
6
2020
medline:
20
6
2020
Statut:
epublish
Résumé
In the present study, it was aimed to study the antioxidant effects of spironolactone (SPL) to determine its possible protective effects in hepatic ischemia reperfusion injury. Hepatic artery, portal vein, and bile duct of Wistar albino rats were clamped for 45 minutes under anesthesia to form an ischemia period. Then reperfusion was allowed and the rats were decapitated 60 minutes later. SPL (20 mg/kg, p.o.) or SF was orally administered for 30 minutes before ischemia. Rats in the control arm underwent sham surgery and were administered isotonic saline. Liver function was studied by measuring aspartate aminotransferase (AST), alanine aminotransferase (ALT), tumor necrosis factor-alpha (TNF-α), and interleukin 1beta (IL-1β) levels. Malondialdehyde (MDA), glutathione (GSH), luminol, and lucigenin levels, myeloperoxidase (MPO) and Na+-K+-ATPase enzyme activities were analyzed to study tissue injury under light microscope. While IR increased AST, ALT, TNF-α, and IL-1β levels and MDA, luminol, and lusigenin levels and MPO activities, it caused a decrease in GSH levels and Na+K+-ATPase activity. Spironolactone administration significantly improved these values. Protective effects of SPL against ischemia/reperfusion injury via various mechanisms suggest that this agent may become a novel treatment agent in clinical practice.
Identifiants
pubmed: 32551425
doi: 10.5578/turkjsurg.4340
pmc: PMC7282454
doi:
Types de publication
Journal Article
Langues
eng
Pagination
285-292Informations de copyright
Copyright © 2019, Turkish Surgical Society.
Déclaration de conflit d'intérêts
Conflict of Interest: The authors have no conflicts of interest to declare.
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