Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing.
COVID 19
SARS-CoV-2
heat treatment
molecular methods
nasopharyngeal samples
rt-Real time PCR
Journal
Emerging microbes & infections
ISSN: 2222-1751
Titre abrégé: Emerg Microbes Infect
Pays: United States
ID NLM: 101594885
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
entrez:
20
6
2020
pubmed:
20
6
2020
medline:
25
6
2020
Statut:
ppublish
Résumé
The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
Identifiants
pubmed: 32552549
doi: 10.1080/22221751.2020.1775500
pmc: PMC7473159
doi:
Substances chimiques
RNA, Viral
0
Viral Proteins
0
Types de publication
Evaluation Study
Letter
Langues
eng
Sous-ensembles de citation
IM
Pagination
1393-1396Références
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