Single tube multiplex PCR assay for the identification of banned meat species.
Animals
Buffaloes
/ genetics
Cattle
/ genetics
Chickens
/ genetics
DNA
/ analysis
Dogs
/ genetics
Equidae
/ genetics
Food Contamination
/ analysis
Goats
/ genetics
Horses
/ genetics
Meat
/ analysis
Multiplex Polymerase Chain Reaction
/ methods
RNA, Ribosomal
/ genetics
Sensitivity and Specificity
Sheep
/ genetics
Swine
/ genetics
12S r RNA
Multiplex PCR
Pakistan
admixture
adulteration
meat species
Journal
Food additives & contaminants. Part B, Surveillance
ISSN: 1939-3229
Titre abrégé: Food Addit Contam Part B Surveill
Pays: England
ID NLM: 101317183
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
pubmed:
20
6
2020
medline:
16
7
2021
entrez:
20
6
2020
Statut:
ppublish
Résumé
Food adulteration has a direct impact on public health, religious faith, fair-trades, and wildlife. In the present study, a reliable and sensitive assay has been developed for verifying meat adulteration in food chain. The multiplex PCR system was optimised for identification of chicken, cow/buffalo, sheep/goat, horse/donkey, pork, and dog DNAs in a single reaction mixture simultaneously. The primers were designed using
Identifiants
pubmed: 32552602
doi: 10.1080/19393210.2020.1778098
doi:
Substances chimiques
RNA, Ribosomal
0
RNA, ribosomal, 12S
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM