Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.
ARL8
BORC
axonal transport
corpus callosum
hippocampus
kinesins
lysosomes
microtubules
neuromuscular junction
synaptic vesicle precursors
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
16 06 2020
16 06 2020
Historique:
received:
12
11
2019
revised:
07
04
2020
accepted:
26
05
2020
entrez:
20
6
2020
pubmed:
20
6
2020
medline:
4
6
2021
Statut:
ppublish
Résumé
BORC is a multisubunit complex previously shown to promote coupling of mammalian lysosomes and C. elegans synaptic vesicle (SV) precursors (SVPs) to kinesins for anterograde transport of these organelles along microtubule tracks. We attempted to meld these observations into a unified model for axonal transport in mammalian neurons by testing two alternative hypotheses: (1) that SV and lysosomal proteins are co-transported within a single type of "lysosome-related vesicle" and (2) that SVPs and lysosomes are distinct organelles, but both depend on BORC for axonal transport. Analyses of various types of neurons from wild-type rats and mice, as well as from BORC-deficient mice, show that neither hypothesis is correct. We find that SVPs and lysosomes are transported separately, but only lysosomes depend on BORC for axonal transport in these neurons. These findings demonstrate that SVPs and lysosomes are distinct organelles that rely on different machineries for axonal transport in mammalian neurons.
Identifiants
pubmed: 32553155
pii: S2211-1247(20)30755-5
doi: 10.1016/j.celrep.2020.107775
pmc: PMC7478246
mid: NIHMS1604811
pii:
doi:
Substances chimiques
Proteins
0
lysosomal proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
107775Subventions
Organisme : Intramural NIH HHS
ID : Z01 HD001607
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA HD001607
Pays : United States
Informations de copyright
Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
Références
PLoS Genet. 2014 Oct 16;10(10):e1004644
pubmed: 25329901
Nat Rev Mol Cell Biol. 2020 Feb;21(2):101-118
pubmed: 31768005
Mol Biol Cell. 2004 Aug;15(8):3729-39
pubmed: 15155810
J Neurosci. 2011 May 25;31(21):7817-30
pubmed: 21613495
Neuron. 2001 Dec 6;32(5):787-800
pubmed: 11738026
Curr Biol. 2019 Jan 21;29(2):268-282.e8
pubmed: 30612907
Nat Genet. 2013 Jun;45(6):639-47
pubmed: 23603762
Dev Cell. 2011 Dec 13;21(6):1171-8
pubmed: 22172677
J Biochem. 2002 Sep;132(3):483-91
pubmed: 12204119
Cell. 1987 Jun 5;49(5):669-77
pubmed: 3107839
J Biol Chem. 1991 Feb 15;266(5):3239-45
pubmed: 1993697
Cell Rep. 2019 Jul 2;28(1):51-64.e4
pubmed: 31269450
J Cell Biol. 2017 Dec 4;216(12):4141-4151
pubmed: 28978644
Neurosci Lett. 2019 Apr 1;697:1-9
pubmed: 29626653
Neuron. 1997 Apr;18(4):613-22
pubmed: 9136770
PLoS Genet. 2017 Nov 16;13(11):e1007100
pubmed: 29145394
J Cell Biol. 2017 Dec 4;216(12):4199-4215
pubmed: 28993467
Traffic. 2019 Jun;20(6):404-435
pubmed: 30945407
Cell Rep. 2018 Jul 31;24(5):1254-1265
pubmed: 30067980
J Cell Biol. 1995 Nov;131(4):1039-53
pubmed: 7490281
BMC Med Genomics. 2016 Jul 19;9(1):42
pubmed: 27435318
Cell. 1985 Jul;41(3):1017-28
pubmed: 3924408
Cell. 2001 Jun 1;105(5):587-97
pubmed: 11389829
Cell Rep. 2016 Aug 23;16(8):2129-2141
pubmed: 27524618
Hum Mol Genet. 2015 Dec 15;24(24):6877-85
pubmed: 26385635
Traffic. 2004 Mar;5(3):140-51
pubmed: 15086790
Genomics. 1998 Sep 1;52(2):209-13
pubmed: 9782088
J Neurochem. 2012 Jun;121(6):861-80
pubmed: 22404429
Nat Rev Mol Cell Biol. 2009 Oct;10(10):682-96
pubmed: 19773780
Mol Biol Cell. 2005 Sep;16(9):4231-42
pubmed: 15987739
Neuron. 2013 Jun 19;78(6):994-1011
pubmed: 23727120
J Cell Biol. 1981 Sep;90(3):675-86
pubmed: 6169734
Am J Hum Genet. 2002 Nov;71(5):1189-94
pubmed: 12355402
Traffic. 2018 May;19(5):354-369
pubmed: 29451726
Mol Biol Cell. 2005 Feb;16(2):483-96
pubmed: 15563606
Neuron. 2018 Sep 19;99(6):1216-1232.e7
pubmed: 30174114
J Neurosci. 2004 Jul 21;24(29):6629-37
pubmed: 15269275
Genetics. 2018 Mar;208(3):1131-1146
pubmed: 29339408
J Cell Biol. 1985 Jul;101(1):85-95
pubmed: 2409098
J Neurosci. 2001 Jun 1;21(11):3830-8
pubmed: 11356871
Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2955-E2964
pubmed: 28320970
Traffic. 2003 Nov;4(11):785-801
pubmed: 14617360
PLoS Biol. 2019 May 17;17(5):e3000279
pubmed: 31100061
Methods Mol Biol. 2016;1496:13-30
pubmed: 27631998
Neuron. 2014 Oct 22;84(2):292-309
pubmed: 25374356
Cell. 1995 Jun 2;81(5):769-80
pubmed: 7539720
J Cell Biol. 1995 Dec;131(6 Pt 2):1801-9
pubmed: 8557746
Cell Microbiol. 2011 Nov;13(11):1812-23
pubmed: 21824248
J Neurosci. 2000 Sep 1;20(17):6374-84
pubmed: 10964943
Neuron. 2003 Dec 4;40(5):945-57
pubmed: 14659093
Curr Biol. 2017 Sep 11;27(17):2569-2578.e4
pubmed: 28823680
Neuron. 2018 Mar 21;97(6):1268-1283.e6
pubmed: 29566793
Nat Cell Biol. 2008 Nov;10(11):1269-79
pubmed: 18849981
Cell. 2010 Apr 16;141(2):290-303
pubmed: 20381137
Neuron. 2010 Jun 10;66(5):710-23
pubmed: 20547129
Neuron. 2019 Oct 23;104(2):239-255.e12
pubmed: 31422865
Cell. 1998 Jun 26;93(7):1147-58
pubmed: 9657148
Autophagy. 2017 Oct 3;13(10):1648-1663
pubmed: 28825857
J Cell Biol. 2018 Sep 3;217(9):3127-3139
pubmed: 29695488
Genome Res. 2011 May;21(5):658-64
pubmed: 21487076
Am J Hum Genet. 2011 Aug 12;89(2):219-30
pubmed: 21820098
Trends Cell Biol. 2016 Feb;26(2):121-134
pubmed: 26541125
Cell. 2019 Jan 10;176(1-2):56-72.e15
pubmed: 30612743
Ann Clin Transl Neurol. 2015 Jun;2(6):623-35
pubmed: 26125038
Cell Rep. 2015 Nov 10;13(6):1221-1232
pubmed: 26527003
Cell. 1992 Sep 4;70(5):861-7
pubmed: 1355409
Nat Genet. 2003 Apr;33(4):455-6
pubmed: 12627231
Neuron. 2019 Jun 19;102(6):1184-1198.e10
pubmed: 31072786
Mol Biol Cell. 2001 Aug;12(8):2275-89
pubmed: 11514616
Dev Cell. 2012 Jul 17;23(1):166-80
pubmed: 22705394
Science. 2000 Aug 11;289(5481):957-60
pubmed: 10938000
Dev Cell. 2015 Apr 20;33(2):176-88
pubmed: 25898167
Stem Cell Reports. 2017 Oct 10;9(4):1221-1233
pubmed: 28966121
Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5862-7
pubmed: 22451907
Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):E3699-708
pubmed: 26124111
Traffic. 2020 Jan;21(1):138-155
pubmed: 31603614
Cell. 1992 Aug 21;70(4):539-51
pubmed: 1505023
Curr Opin Neurobiol. 2019 Aug;57:94-101
pubmed: 30784982
Nat Cell Biol. 2004 Oct;6(10):941-53
pubmed: 15459722
Ann Neurol. 2016 Oct;80(4):633-7
pubmed: 27463701
J Biol Chem. 2004 Jul 2;279(27):28393-401
pubmed: 15102850
Cell Rep. 2016 Nov 15;17(8):1950-1961
pubmed: 27851960
Elife. 2013 Mar 06;2:e00133
pubmed: 23482306
J Cell Biol. 2017 Dec 4;216(12):4183-4197
pubmed: 28993468
Cell. 2019 Sep 19;179(1):147-164.e20
pubmed: 31539493
J Cell Biol. 2003 Apr 14;161(1):55-66
pubmed: 12682084
Curr Opin Neurobiol. 2008 Oct;18(5):495-503
pubmed: 18950710