The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte.
Animals
Blood Coagulation
/ genetics
Cattle
Chromatography, High Pressure Liquid
Complement C3
/ analysis
Cumulus Cells
/ metabolism
Female
Follicular Fluid
/ metabolism
In Vitro Oocyte Maturation Techniques
Oocytes
/ growth & development
Protein Interaction Maps
/ genetics
Proteome
/ analysis
Receptors, Cell Surface
/ genetics
Tandem Mass Spectrometry
Time Factors
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 06 2020
18 06 2020
Historique:
received:
18
12
2019
accepted:
26
05
2020
entrez:
20
6
2020
pubmed:
20
6
2020
medline:
15
12
2020
Statut:
epublish
Résumé
In vitro maturation (IVM) of oocytes has still a negative impact on the developmental competence of oocytes. Therefore, this study analysed the cumulus proteome of individual cumulus-oocyte complexes (COCs) with and without maturational competence, matured under in vivo or in vitro conditions (n = 5 per group). A novel, ultrasensitive mass spectrometry (MS) based protein profiling approach, using label-free quantification, was applied. The detected cumulus proteome included 2226 quantifiable proteins and was highly influenced by the maturation condition (479 differentially expressed proteins) as well as maturational competence of the corresponding oocyte (424 differentially expressed proteins). Enrichment analysis showed an overrepresentation of the complement and coagulation cascades (CCC), ECM-receptor interaction and steroid biosynthesis in cumulus of COCs that matured successfully under in vivo conditions. Verification of the origin of CCC proteins was achieved through detection of C3 secretion into the maturation medium, with significantly increasing concentrations from 12 (48.4 ng/ml) to 24 hours (68 ng/ml: p < 0.001). In relation, concentrations in follicular fluid, reflecting the in vivo situation, were >100x higher. In summary, this study identified important pathways that are impaired in IVM cumulus, as well as potential markers of the maturational competence of oocytes.
Identifiants
pubmed: 32555221
doi: 10.1038/s41598-020-66822-z
pii: 10.1038/s41598-020-66822-z
pmc: PMC7303117
doi:
Substances chimiques
Complement C3
0
Proteome
0
Receptors, Cell Surface
0
extracellular matrix receptor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9880Références
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