Metformin protects against mouse oocyte apoptosis defects induced by arecoline.
apoptosis
arecoline
cytoskeleton
metformin
mitochondrion
oocyte
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
23
08
2019
revised:
19
03
2020
accepted:
24
03
2020
pubmed:
20
6
2020
medline:
4
8
2020
entrez:
20
6
2020
Statut:
ppublish
Résumé
Arecoline is the main bioactive substance extracted from Areca catechu L, which has cell, neural and genetic toxicity. The function of arecoline in reproductive system has not been well explored. To investigate the toxic effects of arecoline on oocyte development, immunofluorescence staining, qPCR, Western blotting, sperm binding assays and in vitro fertilization were performed to evaluate oocyte meiosis competence and embryo development. Our data revealed that arecoline exposure disrupts actin filament dynamics, spindle assembly and kinetochore-microtubule attachment stability in mouse oocytes, leading to aneuploidy and oocyte meiosis arrest. In addition, arecoline treatment disturbs the distribution of mitochondria, reduces ATP production and increases the level of oxidative stress, which ultimately induces oocyte apoptosis. Supplementation with metformin, a medicine for type 2 diabetes in the clinic, partially alleviates these damages. Metformin has a protective effect on arecoline-induced mouse oocytes apoptosis.
Identifiants
pubmed: 32557964
doi: 10.1111/cpr.12809
pmc: PMC7377942
doi:
Substances chimiques
Protective Agents
0
Arecoline
4ALN5933BH
Metformin
9100L32L2N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12809Subventions
Organisme : High-level Personnel Scientific Research Fund of Qingdao Agricultural University
ID : 665-1117004
Organisme : High-level Personnel Scientific Research Fund of Qingdao Agricultural University
ID : 665-1118010
Informations de copyright
© 2020 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
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