Immunogenicity and Efficacy of Zika Virus Envelope Domain III in DNA, Protein, and ChAdOx1 Adenoviral-Vectored Vaccines.
DENV
EDIII
ZIKV
adenovirus
vaccine
Journal
Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355
Informations de publication
Date de publication:
16 Jun 2020
16 Jun 2020
Historique:
received:
21
05
2020
revised:
09
06
2020
accepted:
11
06
2020
entrez:
21
6
2020
pubmed:
21
6
2020
medline:
21
6
2020
Statut:
epublish
Résumé
The flavivirus envelope protein domain III (EDIII) was an effective immunogen against dengue virus (DENV) and other related flaviviruses. Whether this can be applied to the Zika virus (ZIKV) vaccinology remains an open question. Here, we tested the efficacy of ZIKV-EDIII against ZIKV infection, using several vaccine platforms that present the antigen in various ways. We provide data demonstrating that mice vaccinated with a ZIKV-EDIII as DNA or protein-based vaccines failed to raise fully neutralizing antibodies and did not control viremia, following a ZIKV challenge, despite eliciting robust antibody responses. Furthermore, we showed that ZIKV-EDIII encoded in replication-deficient Chimpanzee adenovirus (ChAdOx1-EDIII) elicited anti-ZIKV envelope antibodies in vaccinated mice but also provided limited protection against ZIKV in two physiologically different mouse challenge models. Taken together, our data indicate that contrary to what was shown for other flaviviruses like the dengue virus, which has close similarities with ZIKV-EDIII, this antigen might not be a suitable vaccine candidate for the correct induction of protective immune responses against ZIKV.
Identifiants
pubmed: 32560145
pii: vaccines8020307
doi: 10.3390/vaccines8020307
pmc: PMC7350260
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Medical Research Council
ID : MC_U130184144
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12014/2
Pays : United Kingdom
Organisme : Innovate UK
ID : 972216
Organisme : Department of Health, Social Services and Public Safety, UK Government
ID : 16/107/05
Références
Structure. 2012 Feb 8;20(2):303-14
pubmed: 22285214
Infect Genet Evol. 2016 Sep;43:43-9
pubmed: 27156653
Protein Eng. 1997 Jun;10(6):731-6
pubmed: 9278288
J Virol. 2006 Jul;80(14):6982-92
pubmed: 16809304
J Virol. 2018 Aug 29;92(18):
pubmed: 29976679
J Clin Virol. 2015 Mar;64:160-73
pubmed: 25453327
PLoS One. 2017 Jul 25;12(7):e0181734
pubmed: 28742857
JCI Insight. 2017 Jun 15;2(12):
pubmed: 28614803
Cell. 2016 Aug 11;166(4):1016-1027
pubmed: 27475895
Virol J. 2018 Dec 27;15(1):193
pubmed: 30587198
Front Immunol. 2018 Aug 23;9:1919
pubmed: 30190720
J Gen Virol. 2015 Nov;96(11):3265-3279
pubmed: 26358704
Nat Med. 2005 May;11(5):522-30
pubmed: 15852016
Nat Rev Microbiol. 2013 Feb;11(2):115-28
pubmed: 23321534
Virol J. 2013 Apr 24;10:129
pubmed: 23617954
Vaccines (Basel). 2019 Jul 23;7(3):
pubmed: 31340594
Nature. 2016 Aug 4;536(7614):48-53
pubmed: 27338953
Virology. 2011 Mar 15;411(2):306-15
pubmed: 21255816
Nature. 2016 Apr 19;533(7603):425-8
pubmed: 27093288
Development. 2017 Nov 15;144(22):4114-4124
pubmed: 28993398
Viruses. 2017 Apr 30;9(5):
pubmed: 28468299
Nature. 2005 Sep 29;437(7059):764-9
pubmed: 16193056
Vaccine. 2017 Jul 24;35(33):4287-4294
pubmed: 28669618
Lancet. 2015 Jan 31;385(9966):453-65
pubmed: 25230594
Cell Host Microbe. 2017 Feb 8;21(2):134-142
pubmed: 28182948
J Virol. 2006 Dec;80(24):12149-59
pubmed: 17035317
Vaccine. 2009 Oct 9;27(43):6011-21
pubmed: 19665609
Nature. 2016 Aug 25;536(7617):474-8
pubmed: 27355570
PLoS Negl Trop Dis. 2015 Jul 28;9(7):e0003947
pubmed: 26218926
Vaccine. 2005 May 16;23(26):3469-76
pubmed: 15837370
Immunol Rev. 2008 Oct;225:212-25
pubmed: 18837784
J Virol. 2001 Apr;75(8):4002-7
pubmed: 11264392
Antiviral Res. 2016 Jun;130:69-80
pubmed: 26996139
Nature. 2016 Dec 15;540(7633):443-447
pubmed: 27819683
Lancet. 2017 Nov 4;390(10107):2099-2109
pubmed: 28647173
Sci Rep. 2017 Aug 9;7(1):7679
pubmed: 28794424
Science. 2016 Apr 22;352(6284):467-70
pubmed: 27033547
Cell Host Microbe. 2008 Sep 11;4(3):229-38
pubmed: 18779049
Nat Commun. 2018 Jun 22;9(1):2441
pubmed: 29934593
Structure. 2004 Sep;12(9):1607-18
pubmed: 15341726
Cell Host Microbe. 2010 Sep 16;8(3):271-83
pubmed: 20833378
Vaccine. 2009 Oct 30;27(46):6444-53
pubmed: 19580892
Nature. 2016 May 11;534(7606):267-71
pubmed: 27279226
Appl Microbiol Biotechnol. 2018 Apr;102(7):2977-2996
pubmed: 29470620
J Virol. 2002 Dec;76(24):13097-100
pubmed: 12438639
Cell Host Microbe. 2016 May 11;19(5):696-704
pubmed: 27158114
Emerg Microbes Infect. 2017 Oct 11;6(10):e89
pubmed: 29018252
PLoS Negl Trop Dis. 2016 May 05;10(5):e0004658
pubmed: 27149521