3D Printed Monolithic Microreactors for Real-Time Detection of
3D printing
antibiotic resistance
recombinase polymerase amplification (RPA)
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
17 Jun 2020
17 Jun 2020
Historique:
received:
02
06
2020
revised:
15
06
2020
accepted:
16
06
2020
entrez:
21
6
2020
pubmed:
21
6
2020
medline:
21
6
2020
Statut:
epublish
Résumé
We investigate the compatibility of three 3D printing materials towards real-time recombinase polymerase amplification (rtRPA). Both the general ability of the rtRPA reaction to occur while in contact with the cured 3D printing materials as well as the residual autofluorescence and fluorescence drift in dependence on post curing of the materials is characterized. We 3D printed monolithic rtRPA microreactors and subjected the devices to different post curing protocols. Residual autofluorescence and drift, as well as rtRPA kinetics, were then measured in a custom-made mobile temperature-controlled fluorescence reader (mTFR). Furthermore, we investigated the effects of storage on the devices over a 30-day period. Finally, we present the single- and duplex rtRPA detection of both the organism-specific
Identifiants
pubmed: 32560308
pii: mi11060595
doi: 10.3390/mi11060595
pmc: PMC7344889
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : European Regional Development Fund
ID : 85017408
Organisme : Bundesministerium für Bildung und Forschung
ID : 16GW0145
Organisme : Brandenburger Staatsministerium für Wissenschaft, Forschung und Kultur
ID : 06-GeCa:H228-05/002/004
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