Mineralized DNA-collagen complex-based biomaterials for bone tissue engineering.
Biocompatible Materials
/ chemistry
Bone Regeneration
Cell Survival
Cells, Cultured
Chemical Phenomena
Collagen
/ chemistry
DNA
/ chemistry
Durapatite
Humans
Macromolecular Substances
/ chemistry
Minerals
/ chemistry
Osteoblasts
/ cytology
Spectrum Analysis
Tissue Engineering
Tissue Scaffolds
/ chemistry
DNA-collagen complex
Self-assembly
Tissue engineering
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Oct 2020
15 Oct 2020
Historique:
received:
11
05
2020
revised:
03
06
2020
accepted:
12
06
2020
pubmed:
21
6
2020
medline:
30
3
2021
entrez:
21
6
2020
Statut:
ppublish
Résumé
DNA is a highly polyanionic biomolecule that complexes with both collagen and hydroxyapatite. By combining these complexes, we synthesized nucleic-acid collagen complexes (NACC) mineralized with hydroxyapatite. The composite complexes were made using a short, monodisperse single-stranded DNA, type I collagen, and mineralizing medium. They rapidly self-assembled into both mineralized NACC microfibers and 3D NACC gels. At the nanoscale, these complexes are hierarchical, interwoven, curly nanofibrils resembling native extracellular matrix, which mineralized an interpenetrating nanocrystalline hydroxyapatite phase. Mineralization was able to be done either before or after NACC formation enabling temporal control of the process. In response to the NACC material, primary human osteoblasts took on an osteocyte-like morphology. Moreover, the cells agglomerated and remodeled the NACC gels into densified, tissue-like structures within 3 days. NACC fibers and gels have promise not only as osteoconductive coatings and scaffolds, but as coatings and scaffolds for any tissue using this new form of naturally-derived biomaterials.
Identifiants
pubmed: 32561285
pii: S0141-8130(20)33564-9
doi: 10.1016/j.ijbiomac.2020.06.126
pmc: PMC7494536
mid: NIHMS1604009
pii:
doi:
Substances chimiques
Biocompatible Materials
0
Macromolecular Substances
0
Minerals
0
Collagen
9007-34-5
DNA
9007-49-2
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1127-1139Subventions
Organisme : NHLBI NIH HHS
ID : F31 HL147445
Pays : United States
Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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