Non-coding RNAs in cancer: platforms and strategies for investigating the genomic "dark matter".
Cancer genetics
Long-non coding RNAs
Non-coding RNAs
lncRNAs
miRNAs
microRNAs
ncRNA functions
Journal
Journal of experimental & clinical cancer research : CR
ISSN: 1756-9966
Titre abrégé: J Exp Clin Cancer Res
Pays: England
ID NLM: 8308647
Informations de publication
Date de publication:
20 Jun 2020
20 Jun 2020
Historique:
received:
06
05
2020
accepted:
11
06
2020
entrez:
22
6
2020
pubmed:
22
6
2020
medline:
6
3
2021
Statut:
epublish
Résumé
The discovery of the role of non-coding RNAs (ncRNAs) in the onset and progression of malignancies is a promising frontier of cancer genetics. It is clear that ncRNAs are candidates for therapeutic intervention, since they may act as biomarkers or key regulators of cancer gene network. Recently, profiling and sequencing of ncRNAs disclosed deep deregulation in human cancers mostly due to aberrant mechanisms of ncRNAs biogenesis, such as amplification, deletion, abnormal epigenetic or transcriptional regulation. Although dysregulated ncRNAs may promote hallmarks of cancer as oncogenes or antagonize them as tumor suppressors, the mechanisms behind these events remain to be clarified. The development of new bioinformatic tools as well as novel molecular technologies is a challenging opportunity to disclose the role of the "dark matter" of the genome. In this review, we focus on currently available platforms, computational analyses and experimental strategies to investigate ncRNAs in cancer. We highlight the differences among experimental approaches aimed to dissect miRNAs and lncRNAs, which are the most studied ncRNAs. These two classes indeed need different investigation taking into account their intrinsic characteristics, such as length, structures and also the interacting molecules. Finally, we discuss the relevance of ncRNAs in clinical practice by considering promises and challenges behind the bench to bedside translation.
Identifiants
pubmed: 32563270
doi: 10.1186/s13046-020-01622-x
pii: 10.1186/s13046-020-01622-x
pmc: PMC7305591
doi:
Substances chimiques
Biomarkers, Tumor
0
RNA, Untranslated
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
117Subventions
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 21588
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 9980
Organisme : CARICAL foundation
ID : 16695
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