Human placenta-derived mesenchymal stem cells attenuate established hyperoxia-induced lung injury in newborn rats.
hyperoxia
lung injury
mesenchymal stem cells
sonic hedgehog pathway
Journal
Pediatrics and neonatology
ISSN: 2212-1692
Titre abrégé: Pediatr Neonatol
Pays: Singapore
ID NLM: 101484755
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
13
11
2019
revised:
06
03
2020
accepted:
26
05
2020
pubmed:
23
6
2020
medline:
7
4
2021
entrez:
23
6
2020
Statut:
ppublish
Résumé
Hyperoxia increases Sonic hedgehog (Shh) expression in neonatal rat lungs. The effect of mesenchymal stem cells (MSCs) on the hedgehog signaling pathway in hyperoxia-induced lung injury is unknown. This study evaluated whether MSCs could inhibit hedgehog signaling and improve established hyperoxia-induced lung injury in newborn rats. Newborn rats were assigned to room air (RA) or hyperoxia (85% O Neonatal hyperoxia on postnatal days 4-15 reduced the body weight, increased the mean linear intercept, and decreased the vascular density of the rats, and these effects were associated with increased Shh and Smoothened (Smo) expression and decreased Patched expression. By contrast, the MSC-treated hyperoxic pups exhibited improved alveolarization, increased vascularization, and decreased Shh and Smo expression on postnatal day 29. Human MSC treatment reversed established hyperoxia-induced lung injury in newborn rats, probably through suppression of the hedgehog pathway.
Sections du résumé
BACKGROUND
Hyperoxia increases Sonic hedgehog (Shh) expression in neonatal rat lungs. The effect of mesenchymal stem cells (MSCs) on the hedgehog signaling pathway in hyperoxia-induced lung injury is unknown. This study evaluated whether MSCs could inhibit hedgehog signaling and improve established hyperoxia-induced lung injury in newborn rats.
METHODS
Newborn rats were assigned to room air (RA) or hyperoxia (85% O
RESULTS
Neonatal hyperoxia on postnatal days 4-15 reduced the body weight, increased the mean linear intercept, and decreased the vascular density of the rats, and these effects were associated with increased Shh and Smoothened (Smo) expression and decreased Patched expression. By contrast, the MSC-treated hyperoxic pups exhibited improved alveolarization, increased vascularization, and decreased Shh and Smo expression on postnatal day 29.
CONCLUSION
Human MSC treatment reversed established hyperoxia-induced lung injury in newborn rats, probably through suppression of the hedgehog pathway.
Identifiants
pubmed: 32564932
pii: S1875-9572(20)30090-5
doi: 10.1016/j.pedneo.2020.05.012
pii:
doi:
Substances chimiques
Hedgehog Proteins
0
Shh protein, rat
0
Smo protein, rat
0
Smoothened Receptor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
498-505Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None.