Sphingosine 1-phosphate lyase blockade elicits myogenic differentiation of murine myoblasts acting via Spns2/S1P
Myogenic differentiation
Sphingosine 1-phosphate
Journal
Biochimica et biophysica acta. Molecular and cell biology of lipids
ISSN: 1879-2618
Titre abrégé: Biochim Biophys Acta Mol Cell Biol Lipids
Pays: Netherlands
ID NLM: 101731727
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
28
02
2020
revised:
29
05
2020
accepted:
14
06
2020
pubmed:
23
6
2020
medline:
1
1
2021
entrez:
23
6
2020
Statut:
ppublish
Résumé
The bioactive sphingolipid sphingosine 1-phosphate (S1P) has emerged in the last three decades as main regulator of key cellular processes including cell proliferation, survival, migration and differentiation. A crucial role for this sphingolipid has been recognized in skeletal muscle cell biology both in vitro and in vivo. S1P lyase (SPL) is responsible for the irreversible degradation of S1P and together with sphingosine kinases, the S1P producing enzymes, regulates cellular S1P levels. In this study is clearly showed that the blockade of SPL by pharmacological or RNA interference approaches induces myogenic differentiation of C2C12 myoblasts. Moreover, down-regulation of the specific S1P transporter spinster homolog 2 (Spns2) abrogates myogenic differentiation brought about by SPL inhibition or down-regulation, pointing at a role of extracellular S1P in the pro-myogenic action induced by SPL blockade. Furthermore, also S1P
Identifiants
pubmed: 32565314
pii: S1388-1981(20)30151-7
doi: 10.1016/j.bbalip.2020.158759
pii:
doi:
Substances chimiques
Anion Transport Proteins
0
Sphingosine-1-Phosphate Receptors
0
Spns2 protein, mouse
0
Aldehyde-Lyases
EC 4.1.2.-
sphingosine 1-phosphate lyase (aldolase)
EC 4.1.2.27
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
158759Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.