Integration of proteome and transcriptome refines key molecular processes underlying oil production in
Lipid biosynthesis
Oleaginous algae
Photosynthesis
Triacylglycerols
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
2020
2020
Historique:
received:
20
12
2019
accepted:
08
06
2020
entrez:
23
6
2020
pubmed:
23
6
2020
medline:
23
6
2020
Statut:
epublish
Résumé
Under nitrogen deficiency situation, Physiological adaptations to N- not apparent from transcriptome data were unveiled: (a) abundance of proteins related to photosynthesis only slightly decreased in the first 48 h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48 h under N-, suggesting that respiration is enhanced after 48 h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48 h, synchronous with the previously reported diversification of fatty acids after 48 h. This study adds a proteome perspective on the major pathways for TAG accumulation in
Sections du résumé
BACKGROUND
BACKGROUND
Under nitrogen deficiency situation,
RESULTS
RESULTS
Physiological adaptations to N- not apparent from transcriptome data were unveiled: (a) abundance of proteins related to photosynthesis only slightly decreased in the first 48 h, indicating that photosynthesis is still working efficiently, and protein amounts adjust gradually with reduction in chloroplast size. (b) Most proteins related to the TCA cycle were strongly upregulated after 48 h under N-, suggesting that respiration is enhanced after 48 h and that TCA cycle efflux supports the carbon required for lipid synthesis. (c) Proteins related to lipid accumulation via the Kennedy pathway increased their abundance at 48 h, synchronous with the previously reported diversification of fatty acids after 48 h.
CONCLUSIONS
CONCLUSIONS
This study adds a proteome perspective on the major pathways for TAG accumulation in
Identifiants
pubmed: 32565907
doi: 10.1186/s13068-020-01748-2
pii: 1748
pmc: PMC7302151
doi:
Types de publication
Journal Article
Langues
eng
Pagination
109Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interests.
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