Cell Proteins Interacting with the Human Immunodeficiency Virus in Immunoblotting can be Detected by R5- or X4- Tropic Human Immunodeficiency Virus Particles.
Free human immunodeficiency virus particles
gp160
human immunodeficiency virus
western blot
Journal
International journal of applied & basic medical research
ISSN: 2229-516X
Titre abrégé: Int J Appl Basic Med Res
Pays: India
ID NLM: 101579831
Informations de publication
Date de publication:
Historique:
received:
30
01
2019
revised:
27
03
2019
accepted:
06
01
2020
entrez:
23
6
2020
pubmed:
23
6
2020
medline:
23
6
2020
Statut:
ppublish
Résumé
The present study reported a new immunoblot assay, with revelation by R5- or X4-whole free human immunodeficiency virus (HIV) particles or recombinant gp160. The assay was optimized to identify cell proteins interacting with HIV. Whole cell lysates were prepared from peripheral blood lymphocytes (PBLs), dendritic cells (DC), monocyte-derived macrophage (MDM), and Henrietta Lacks (Hela, wild-type or transfected with DC-specific intracellular adhesion molecule-3-Grabbing Non-Integrin, HeLa) and Human endometrial cells (HEC-1A) lines; HIV particles used were the R5-tropic HIV-1 Experiments with PBL lysates and both viruses demonstrated different bands, including a unique band at 105-117 kDa in addition to nonspecific bands. The 105-117 kDa band migrated at the same level of that observed in controls using total PBL lysate and anti-CD4 mAb for detection and thus likely corresponds to the cluster difference (CD) 4 complex. Blots using lysates of DCs, MDM, HeLa cell line, and HEC-1A cell line allowed identifying several bands that positions were similar to that seen by recombinant gp160 or whole R5- or X4-HIV particles. Blot of whole lysates of various HIV target cells is recognized by free HIV particles and allows identifying a wide range of HIV-interacting cell proteins. Such optimized assay could be useful to recognize new cellular HIV attachment proteins.
Identifiants
pubmed: 32566522
doi: 10.4103/ijabmr.IJABMR_398_18
pii: IJABMR-10-81
pmc: PMC7289202
doi:
Types de publication
Journal Article
Langues
eng
Pagination
81-85Informations de copyright
Copyright: © 2020 International Journal of Applied and Basic Medical Research.
Déclaration de conflit d'intérêts
There are no conflicts of interest.
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