In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging.
MALDI
TIMS
ion mobility separation
lipid isomers
mass spectrometry imaging
Journal
Journal of mass spectrometry : JMS
ISSN: 1096-9888
Titre abrégé: J Mass Spectrom
Pays: England
ID NLM: 9504818
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
21
01
2020
revised:
09
04
2020
accepted:
14
04
2020
entrez:
23
6
2020
pubmed:
23
6
2020
medline:
7
4
2021
Statut:
ppublish
Résumé
The highly diverse chemical structures of lipids make their analysis directly from biological tissue sections extremely challenging. Here, we report the in situ mapping and identification of lipids in a freshwater crustacean Gammarus fossarum using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) in combination with an additional separation dimension using ion mobility spectrometry (IMS). The high-resolution trapped ion mobility spectrometry (TIMS) allowed efficient separation of isobaric/isomeric lipids showing distinct spatial distributions. The structures of the lipids were further characterized by MS/MS analysis. It is demonstrated that MALDI MSI with mobility separation is a powerful tool for distinguishing and localizing isobaric/isomeric lipids.
Substances chimiques
Lipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4531Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-18-CE34-0008
Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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