Vimentin plays an important role in the promotion of breast cancer cell migration and invasion by leucine aminopeptidase 3.
Epithelial–mesenchymal transition (EMT)
Leucine aminopeptidase 3 (LAP3)
Metastasis
Vimentin
Journal
Cytotechnology
ISSN: 0920-9069
Titre abrégé: Cytotechnology
Pays: United States
ID NLM: 8807027
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
01
02
2020
accepted:
20
05
2020
pubmed:
24
6
2020
medline:
24
6
2020
entrez:
24
6
2020
Statut:
ppublish
Résumé
Breast cancer is a common type of cancer in females. Our previous studies indicated that leucine aminopeptidase 3 (LAP3) promotes migration and invasion of breast cancer cells. Vimentin is a mesenchymal marker, and its upregulation represents the promotion of epithelial-mesenchymal transition. In this study, we found that LAP3 and vimentin were highly expressed in breast cancer tissues, and the overexpression of LAP3 in breast cancer cells promoted the expression of vimentin. Western blot analysis indicated that the overexpression of LAP3 upregulated the phosphorylation of Erk1/2. MEK inhibitor PD98059 downregulated the expression of vimentin, matrix metalloproteinase-2/9 (MMP-2/9), and fascin through the inhibition of Erk1/2 activity. We hypothesized that LAP3 promoted tumor migration and invasion by upregulating vimentin. The knockdown of vimentin resulted in the inhibited migration and invasion of MDA-MB-231 and MDA-MB-468 cells. The expression of MMP-2/9 and fascin could also be downregulated. In conclusion, vimentin might play an important role in the promotion of breast cancer metastasis by LAP3.
Identifiants
pubmed: 32572729
doi: 10.1007/s10616-020-00402-x
pii: 10.1007/s10616-020-00402-x
pmc: PMC7548013
doi:
Types de publication
Journal Article
Langues
eng
Pagination
639-647Subventions
Organisme : Project of Shandong Province Higher Educational Science and Technology Program
ID : J17KA255
Organisme : Foundation of Weifang Scientific Committee
ID : 2017YX064
Organisme : University Students Science and Technology Innovation Fund Projec
ID : 201910438027X
Organisme : University Students Science and Technology Innovation Fund Projec
ID : KX2019025
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