Feasibility of a label-free X-ray method to estimate brain amyloid load in small animals.
Alzheimer's disease
Amyloid load estimation
Amyloid plaques
Elastic X-ray scattering
Label-free method
Spectral SAXS
Journal
Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558
Informations de publication
Date de publication:
01 09 2020
01 09 2020
Historique:
received:
31
01
2020
revised:
18
06
2020
accepted:
18
06
2020
pubmed:
24
6
2020
medline:
22
6
2021
entrez:
24
6
2020
Statut:
ppublish
Résumé
Amyloid plaque in the brain is associated with a wide range of neurodegenerative diseases such as Alzheimer's and Parkinson's and defined as aggregates of amyloid fibrils rich in β-sheet structures. We report a label-free method based on small-angle X-ray scattering (SAXS) to estimate amyloid load in an intact mouse head with skull. The method is based on recording and analyzing the X rays elastically scattered from the β-sheets of amyloid plaques in a mouse head at angles smaller than 10° and energies between 30 and 45 keV. The method is demonstrated by acquiring the spectral SAXS data of an amyloid model and an excised head from a wild-type mouse for 600 s. We captured the distinct scattering peaks of the amyloid plaques at momentum transfer (q) of 6 and 13 nm There are no previously described label-free X-ray methods for the estimation of amyloid load in an intact head. We demonstrated the feasibility of a label-free method based on SAXS to potentially estimate brain amyloid in small animals.
Sections du résumé
BACKGROUND
Amyloid plaque in the brain is associated with a wide range of neurodegenerative diseases such as Alzheimer's and Parkinson's and defined as aggregates of amyloid fibrils rich in β-sheet structures.
NEW METHOD
We report a label-free method based on small-angle X-ray scattering (SAXS) to estimate amyloid load in an intact mouse head with skull. The method is based on recording and analyzing the X rays elastically scattered from the β-sheets of amyloid plaques in a mouse head at angles smaller than 10° and energies between 30 and 45 keV. The method is demonstrated by acquiring the spectral SAXS data of an amyloid model and an excised head from a wild-type mouse for 600 s.
RESULTS
We captured the distinct scattering peaks of the amyloid plaques at momentum transfer (q) of 6 and 13 nm
COMPARISON WITH EXISTING METHODS
There are no previously described label-free X-ray methods for the estimation of amyloid load in an intact head.
CONCLUSIONS
We demonstrated the feasibility of a label-free method based on SAXS to potentially estimate brain amyloid in small animals.
Identifiants
pubmed: 32574641
pii: S0165-0270(20)30245-4
doi: 10.1016/j.jneumeth.2020.108822
pii:
doi:
Substances chimiques
Amyloid
0
Amyloid beta-Peptides
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
108822Informations de copyright
Published by Elsevier B.V.