Long Noncoding RNA
CDK inhibitor
INK4 locus
cell cycle
cyclin B1
long noncoding RNA
Journal
Cancer genomics & proteomics
ISSN: 1790-6245
Titre abrégé: Cancer Genomics Proteomics
Pays: Greece
ID NLM: 101188791
Informations de publication
Date de publication:
Historique:
received:
07
04
2020
revised:
19
04
2020
accepted:
20
04
2020
entrez:
25
6
2020
pubmed:
25
6
2020
medline:
12
2
2021
Statut:
ppublish
Résumé
The INK4 locus encodes three important genes p15 Following ANROC silencing in cells by siRNA, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and cell cycle analysis using flow cytometry were performed. ANROC expression was decreased by oncogenic RAS signalling. ANROC knockdown enhanced HeLa cell proliferation and induced cyclin B1 mRNA, which promotes G2/M progression of the cell cycle. Furthermore, flow cytometric analysis revealed that ANROC knockdown increased the percentage of cells in the S and G ANROC functions to suppress cell cycle progression by suppressing cyclin B1 expression, thus inhibiting cell proliferation.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
The INK4 locus encodes three important genes p15
MATERIALS AND METHODS
METHODS
Following ANROC silencing in cells by siRNA, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and cell cycle analysis using flow cytometry were performed.
RESULTS
RESULTS
ANROC expression was decreased by oncogenic RAS signalling. ANROC knockdown enhanced HeLa cell proliferation and induced cyclin B1 mRNA, which promotes G2/M progression of the cell cycle. Furthermore, flow cytometric analysis revealed that ANROC knockdown increased the percentage of cells in the S and G
CONCLUSION
CONCLUSIONS
ANROC functions to suppress cell cycle progression by suppressing cyclin B1 expression, thus inhibiting cell proliferation.
Identifiants
pubmed: 32576587
pii: 17/4/425
doi: 10.21873/cgp.20201
pmc: PMC7367603
doi:
Substances chimiques
CCNB1 protein, human
0
CDKN2B protein, human
0
Cyclin B1
0
Cyclin-Dependent Kinase Inhibitor p15
0
Cyclin-Dependent Kinase Inhibitor p16
0
RNA, Long Noncoding
0
RNA, Small Interfering
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
425-430Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
Références
Tumour Biol. 2016 May;37(5):5657-61
pubmed: 26753962
Mol Cell. 2010 Jun 11;38(5):662-74
pubmed: 20541999
Biochim Biophys Acta. 2002 Mar 14;1602(1):73-87
pubmed: 11960696
Oncotarget. 2014 Apr 30;5(8):2276-92
pubmed: 24810364
Mutat Res. 2005 Aug 25;576(1-2):22-38
pubmed: 15878778
Anticancer Res. 2019 Aug;39(8):4073-4077
pubmed: 31366490
Anticancer Res. 2015 Oct;35(10):5377-82
pubmed: 26408699
Genes Dev. 2007 Mar 1;21(5):525-30
pubmed: 17344414
Oncogene. 2011 Apr 21;30(16):1956-62
pubmed: 21151178
Cell. 2018 Jan 25;172(3):393-407
pubmed: 29373828
Nat Rev Mol Cell Biol. 2006 Sep;7(9):667-77
pubmed: 16921403
Genes Dev. 2007 Jan 1;21(1):49-54
pubmed: 17210787
Science. 1996 Dec 6;274(5293):1672-7
pubmed: 8939849