Profiling the circulating mRNA transcriptome in human liver disease.
biomarkers
circulating transcripts
extracellular vesicles
hepatocellular carcinoma
liver cirrhosis
Journal
Oncotarget
ISSN: 1949-2553
Titre abrégé: Oncotarget
Pays: United States
ID NLM: 101532965
Informations de publication
Date de publication:
09 Jun 2020
09 Jun 2020
Historique:
received:
28
02
2020
accepted:
16
05
2020
entrez:
25
6
2020
pubmed:
25
6
2020
medline:
25
6
2020
Statut:
epublish
Résumé
The human circulation contains cell-free DNA and non-coding microRNA (miRNA). Less is known about the presence of messenger RNA (mRNA). This report profiles the human circulating mRNA transcriptome in people with liver cirrhosis (LC) and hepatocellular carcinoma (HCC) to determine whether mRNA analytes can be used as biomarkers of liver disease. Using RNAseq and RT-qPCR, we investigate circulating mRNA in plasma from HCC and LC patients and demonstrate detection of transcripts representing more than 19,000 different protein coding genes. Remarkably, the circulating mRNA expression levels were similar from person to person over the 21 individuals whose samples were analyzed by RNAseq. Liver derived circulating transcripts such as albumin (
Identifiants
pubmed: 32577166
doi: 10.18632/oncotarget.27617
pii: 27617
pmc: PMC7289528
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2216-2232Déclaration de conflit d'intérêts
CONFLICTS OF INTEREST The authors have declared that no conflicts of interest exists for this work. Timothy Block is on the Board of Directors of Hepion, an early phase company developing therapeutics for hepatitis B and other liver diseases. Cirna therapeutics is a startup company created by The Baruch S. Blumberg Institute developing early detection of cancer diagnostics that plans to license technology described in this work. None of the authors have any ownership in Cirna Therapeutics.
Références
J Hepatol. 2017 Jan;66(1):212-227
pubmed: 27423426
Bioinformatics. 2019 Jun 1;35(11):1966-1967
pubmed: 30346488
Hepatology. 2000 Oct;32(4 Pt 1):796-802
pubmed: 11003625
Expert Opin Biol Ther. 2009 Jun;9(6):703-711
pubmed: 19426115
Cancer Res. 2006 Oct 1;66(19):9453-60
pubmed: 17018600
PLoS One. 2016 Feb 17;11(2):e0149183
pubmed: 26886260
J Immunol Res. 2018 May 24;2018:4602570
pubmed: 29951557
J Clin Invest. 2016 Apr 1;126(4):1208-15
pubmed: 27035812
J Biol Chem. 2015 Feb 20;290(8):4545-51
pubmed: 25568317
Cell Regen (Lond). 2013 Apr 30;2(1):3
pubmed: 25408875
Matrix Biol. 2019 Apr;77:41-57
pubmed: 30098419
Int J Mol Sci. 2014 Sep 30;15(10):17644-66
pubmed: 25272224
Clin Cancer Res. 2008 Nov 15;14(22):7444-51
pubmed: 19010861
Clin Transl Gastroenterol. 2017 Jun 15;8(6):e98
pubmed: 28617447
Hepatology. 1995 Feb;21(2):403-7
pubmed: 7843713
Science. 2015 Jan 23;347(6220):1260419
pubmed: 25613900
Clin Cancer Res. 2004 Mar 1;10(5):1613-7
pubmed: 15014011
BMC Gastroenterol. 2010 May 18;10:46
pubmed: 20482774
PLoS One. 2017 Jan 6;12(1):e0164644
pubmed: 28060806
Bioinformatics. 2014 Nov 1;30(21):3109-14
pubmed: 25064568
Transplantation. 2008 Jan 15;85(1):81-7
pubmed: 18192916
Cell. 2017 Jun 15;169(7):1327-1341.e23
pubmed: 28622513
Clin Cancer Res. 2005 May 1;11(9):3205-9
pubmed: 15867214
BMC Cancer. 2017 Jul 11;17(1):479
pubmed: 28693532
JCI Insight. 2019 Apr 11;5:
pubmed: 30973829
RNA Biol. 2018 Feb 1;15(2):242-250
pubmed: 29219730
Cancer Lett. 2008 May 18;263(2):312-20
pubmed: 18280643
Clin Cancer Res. 2000 Oct;6(10):3823-6
pubmed: 11051224
Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22
pubmed: 18228490
Biochimie. 2018 Dec;155:2-10
pubmed: 29555374
J Transl Med. 2014 Dec 14;12:355
pubmed: 25496700
Int J Oncol. 2014 Dec;45(6):2596-604
pubmed: 25230705
J Hum Genet. 2009 Jul;54(7):392-7
pubmed: 19478819
Nat Commun. 2019 Feb 27;10(1):947
pubmed: 30814508
Int J Clin Oncol. 2011 Dec;16(6):617-22
pubmed: 21526393
J Cell Sci. 2013 May 1;126(Pt 9):1962-8
pubmed: 23444373
Gut. 2002 Apr;50(4):530-4
pubmed: 11889075
Am J Physiol Cell Physiol. 2010 Aug;299(2):C189-202
pubmed: 20463170
Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1123-1128
pubmed: 28096363
Hepatology. 1997 Apr;25(4):896-9
pubmed: 9096594
World J Gastroenterol. 2011 Apr 21;17(15):2037-43
pubmed: 21528084
Cancer Genomics Proteomics. 2012 Jul-Aug;9(4):193-8
pubmed: 22798504
J Biol Chem. 2019 Jul 5;294(27):10407-10414
pubmed: 31097545
Ann N Y Acad Sci. 2006 Sep;1075:204-10
pubmed: 17108213
iScience. 2019 Apr 26;14:199-209
pubmed: 30981115
Clin Chem. 2010 Jan;56(1):82-9
pubmed: 19833836
Clin Cancer Res. 1999 Aug;5(8):1961-5
pubmed: 10473072
PLoS One. 2008;3(11):e3694
pubmed: 19002258
Cell Syst. 2016 Sep 28;3(3):287-301.e3
pubmed: 27641958