Structures of human antibodies bound to SARS-CoV-2 spike reveal common epitopes and recurrent features of antibodies.

COVID-19 Coronavirus ELISA Fab IgG MERS-CoV SARS-CoV SARS-CoV-2 convalescent plasma electron microscopy

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
29 May 2020
Historique:
entrez: 25 6 2020
pubmed: 25 6 2020
medline: 25 6 2020
Statut: epublish

Résumé

Neutralizing antibody responses to coronaviruses focus on the trimeric spike, with most against the receptor-binding domain (RBD). Here we characterized polyclonal IgGs and Fabs from COVID-19 convalescent individuals for recognition of coronavirus spikes. Plasma IgGs differed in their degree of focus on RBD epitopes, recognition of SARS-CoV, MERS-CoV, and mild coronaviruses, and how avidity effects contributed to increased binding/neutralization of IgGs over Fabs. Electron microscopy reconstructions of polyclonal plasma Fab-spike complexes showed recognition of both S1

Identifiants

pubmed: 32577645
doi: 10.1101/2020.05.28.121533
pmc: PMC7302198
pii:
doi:

Types de publication

Preprint

Langues

eng

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

Références

J Pathol. 2015 Jan;235(2):185-95
pubmed: 25270030
Nature. 2020 Aug;584(7819):115-119
pubmed: 32454513
Nat Rev Microbiol. 2013 Dec;11(12):836-48
pubmed: 24217413
J Struct Biol. 2005 Oct;152(1):36-51
pubmed: 16182563
Cell Rep. 2020 Mar 17;30(11):3755-3765.e7
pubmed: 32187547
Nature. 2020 Mar;579(7798):265-269
pubmed: 32015508
Nature. 2020 Aug;584(7821):437-442
pubmed: 32555388
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357
pubmed: 28807998
Pediatr Infect Dis J. 2001 Jan;20(1):63-75
pubmed: 11176570
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
J Virol. 2019 Nov 13;93(23):
pubmed: 31534041
Nucleic Acids Res. 2015 Jan;43(Database issue):D413-22
pubmed: 25378316
Nat Med. 2020 Sep;26(9):1422-1427
pubmed: 32651581
Cell. 2020 Jul 9;182(1):73-84.e16
pubmed: 32425270
Cell Res. 2017 Jan;27(1):119-129
pubmed: 28008928
Emerg Microbes Infect. 2019;8(1):516-530
pubmed: 30938227
Mol Syst Biol. 2011 Oct 11;7:539
pubmed: 21988835
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Nat Commun. 2020 May 4;11(1):2251
pubmed: 32366817
Cell Host Microbe. 2016 Sep 14;20(3):392-405
pubmed: 27631702
J Struct Biol. 2015 Nov;192(2):216-21
pubmed: 26278980
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
J Virol. 2018 Jan 30;92(4):
pubmed: 29093093
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21
pubmed: 20057044
PLoS Pathog. 2010 May 27;6(5):e1000908
pubmed: 20523901
Science. 2020 Aug 7;369(6504):650-655
pubmed: 32571838
Immunity. 2019 Jun 18;50(6):1513-1529.e9
pubmed: 31126879
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Nature. 2003 Nov 27;426(6965):450-4
pubmed: 14647384
J Biol Chem. 2006 Jun 9;281(23):15829-36
pubmed: 16597622
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W299-302
pubmed: 15980475
Nat Commun. 2015 Jul 28;6:7712
pubmed: 26218507
Nat Methods. 2018 Nov;15(11):905-908
pubmed: 30377346
Nat Struct Mol Biol. 2016 Oct;23(10):906-915
pubmed: 27617431
Nat Struct Mol Biol. 2019 Jun;26(6):481-489
pubmed: 31160783
Nature. 2016 Mar 3;531(7592):118-21
pubmed: 26935699
Science. 2020 May 8;368(6491):630-633
pubmed: 32245784
Nature. 2013 Mar 14;495(7440):251-4
pubmed: 23486063
Int J Biol Sci. 2020 Mar 15;16(10):1718-1723
pubmed: 32226289
Nature. 2020 May;581(7807):221-224
pubmed: 32225175
J Virol. 2020 Mar 17;94(7):
pubmed: 31996437
Clin Infect Dis. 2008 Aug 1;47(3):401-9
pubmed: 18558875
Science. 2020 Aug 21;369(6506):956-963
pubmed: 32540903
Bioinformatics. 2018 Dec 1;34(23):4121-4123
pubmed: 29790939
Euro Surveill. 2017 Mar 30;22(13):
pubmed: 28382917
Cell Mol Immunol. 2020 Jun;17(6):647-649
pubmed: 32313207
Proc Natl Acad Sci U S A. 2017 Oct 17;114(42):11157-11162
pubmed: 29073020
Nature. 2016 Mar 3;531(7592):114-117
pubmed: 26855426
Science. 2020 Jun 12;368(6496):1274-1278
pubmed: 32404477
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Adv Virus Res. 2019;105:93-116
pubmed: 31522710
J Struct Biol. 2011 Apr;174(1):11-22
pubmed: 21130884
Structure. 2021 Apr 1;29(4):385-392.e5
pubmed: 33378641
Emerg Microbes Infect. 2020 Dec;9(1):2105-2113
pubmed: 32893735
Elife. 2019 Oct 25;8:
pubmed: 31650956
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Nat Rev Microbiol. 2016 Aug;14(8):523-34
pubmed: 27344959
J Biol Chem. 2006 Nov 10;281(45):34610-6
pubmed: 16954221
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Science. 2020 Mar 27;367(6485):1444-1448
pubmed: 32132184
Emerg Microbes Infect. 2020 Dec;9(1):680-686
pubmed: 32207377
Methods. 2016 May 1;100:25-34
pubmed: 26931650
J Virol. 2008 Apr;82(7):3220-35
pubmed: 18199635
Clin Vaccine Immunol. 2010 Jul;17(7):1055-65
pubmed: 20463105
PLoS One. 2010 Mar 10;5(3):e9490
pubmed: 20224823
Nat Commun. 2017 Apr 10;8:15092
pubmed: 28393837
Nature. 2019 Feb;566(7744):393-397
pubmed: 30664748
Nat Commun. 2020 Mar 27;11(1):1620
pubmed: 32221306
Annu Rev Microbiol. 2019 Sep 8;73:529-557
pubmed: 31226023
Glob Chall. 2017 Jan 10;1(1):33-46
pubmed: 31565258
Immunity. 2020 Jul 14;53(1):98-105.e5
pubmed: 32561270
J Virol. 2017 Oct 25;92(4):
pubmed: 29070693
Science. 2020 Aug 7;369(6504):643-650
pubmed: 32540902
Cell. 2015 Sep 10;162(6):1379-90
pubmed: 26359989
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Viruses. 2020 May 06;12(5):
pubmed: 32384820
Nucleic Acids Res. 2014 Jan;42(Database issue):D1140-6
pubmed: 24214988
J Struct Biol. 2007 Jan;157(1):281-7
pubmed: 16963278
Cell. 2019 Feb 21;176(5):1026-1039.e15
pubmed: 30712865
Sci Rep. 2018 Oct 24;8(1):15701
pubmed: 30356097
J Mol Biol. 2005 Jul 1;350(1):126-44
pubmed: 15907931
Syst Biol. 2010 May;59(3):307-21
pubmed: 20525638
PLoS Pathog. 2012;8(8):e1002859
pubmed: 22876187

Auteurs

Christopher O Barnes (CO)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Anthony P West (AP)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Kathryn E Huey-Tubman (KE)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Magnus A G Hoffmann (MAG)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Naima G Sharaf (NG)

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA.

Pauline R Hoffman (PR)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Nicholas Koranda (N)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Harry B Gristick (HB)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Christian Gaebler (C)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Frauke Muecksch (F)

Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.

Julio C Cetrulo Lorenzi (JC)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Shlomo Finkin (S)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Thomas Hagglof (T)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Arlene Hurley (A)

Hospital Program Direction, The Rockefeller University, New York, NY 10065, USA.

Katrina G Millard (KG)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Yiska Weisblum (Y)

Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.

Fabian Schmidt (F)

Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.

Theodora Hatziioannou (T)

Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.

Paul D Bieniasz (PD)

Laboratory of Retrovirology, The Rockefeller University, New York, NY 10065, USA.
Howard Hughes Medical Institute.

Marina Caskey (M)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Davide F Robbiani (DF)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Michel C Nussenzweig (MC)

Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
Howard Hughes Medical Institute.

Pamela J Bjorkman (PJ)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.

Classifications MeSH