LC-MS/MS Analysis of Thiol-Containing Amino Acids in Exosomal Fraction of Serum.
Journal
Journal of chromatographic science
ISSN: 1945-239X
Titre abrégé: J Chromatogr Sci
Pays: United States
ID NLM: 0173225
Informations de publication
Date de publication:
24 Jul 2020
24 Jul 2020
Historique:
received:
08
01
2020
revised:
11
05
2020
accepted:
02
06
2020
pubmed:
25
6
2020
medline:
2
4
2021
entrez:
25
6
2020
Statut:
ppublish
Résumé
It has been suggested that thiol-containing amino acids could be used as biomarkers for diseases associated with oxidative stress. We investigated the thiol-containing amino acids, homocysteine (Hcy), cysteine (Cys), glutathione (GSH) and γ-glutamylcysteine (γ-GluCys), in commercial human serum by using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after precolumn derivatization with 4-fluoro-7-sulfobenzofurazan. This method was applied to determine the composition of thiol-containing amino acids in exosomes prepared from the serum. Hcy, Cys, GSH and γ-GluCys could be detected in the exosomal fraction, and the ratio of each thiol-containing amino acid was similar to those in the corresponding native serum. Cys (94.76%) was most enriched in the exosomal fraction, followed by GSH (2.97%), γ-GluCys (1.59%) and Hcy (0.68%). These findings suggest that thiol-containing amino acids, Hcy, Cys, GSH and γ-GluCys, are included in exosomes in human serum.
Identifiants
pubmed: 32577740
pii: 5861762
doi: 10.1093/chromsci/bmaa028
doi:
Substances chimiques
Dipeptides
0
Glutathione
GAN16C9B8O
Cysteine
K848JZ4886
gamma-glutamylcysteine
M984VJS48P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
636-640Informations de copyright
© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.