Molecular characterization and identification of Blastocystis and its subtypes from raccoon (Procyon lotor) in north of Iran.
Animals
Base Sequence
Blastocystis
/ classification
Blastocystis Infections
/ epidemiology
DNA, Protozoan
/ genetics
Feces
/ parasitology
Genetic Variation
Genotype
Iran
/ epidemiology
Prevalence
Protozoan Infections, Animal
/ epidemiology
RNA, Ribosomal
/ genetics
Raccoons
/ parasitology
Ribosome Subunits, Small
/ genetics
Blastocystis
Raccoon
Subtyping
Zoonotic transmission
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
04
02
2020
accepted:
10
06
2020
pubmed:
25
6
2020
medline:
23
9
2020
entrez:
25
6
2020
Statut:
ppublish
Résumé
Blastocystis is a zoonotic protozoan parasite frequently identified in the intestinal tract of humans and a vast variety of animals, worldwide. Here, we assessed the prevalence of Blastocystis and its subtypes in stool samples of raccoons. Stool samples from 30 raccoons were collected. Total DNA was extracted, and the barcoding region of the small subunit ribosomal rRNA (SSU rRNA) gene was amplified and sequenced. Specific fragment for Blastocystis was successfully amplified in five samples (16.66%). Sequencing analysis revealed ST1, ST2, and ST3 among 1, 2, and 2 Blastocystis-positive samples. Our results documented the presence of Blastocystis subtypes 1-3 in raccoons. Subtype 1 showed higher similarity to the human isolates of Blastocystis. However, it seems that raccoons may emerge as reservoirs for Blastocystis and may be linked to zoonotic transmission of the protist.
Identifiants
pubmed: 32577820
doi: 10.1007/s00436-020-06770-9
pii: 10.1007/s00436-020-06770-9
doi:
Substances chimiques
DNA, Protozoan
0
RNA, Ribosomal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2741-2745Références
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