Bioinformatics analysis and characterization of a secretory cystatin from Thelohanellus kitauei.
Bioinformatics analysis
Inhibitory activity
Molecular docking
Protease inhibitor
TK-cystatin
Thelohanellus kitauei
Journal
AMB Express
ISSN: 2191-0855
Titre abrégé: AMB Express
Pays: Germany
ID NLM: 101561785
Informations de publication
Date de publication:
23 Jun 2020
23 Jun 2020
Historique:
received:
02
05
2020
accepted:
15
06
2020
entrez:
25
6
2020
pubmed:
25
6
2020
medline:
25
6
2020
Statut:
epublish
Résumé
Thelohanellus kitauei, is a member of obligate parasitic myxozoans, which causes intestinal giant-cystic disease of common carp (Cyprinus carpio) and has resulted in significant economic losses in carp farms. Cystatin secreted by parasites can regulate the immune response of host to facilitate parasite's survival. In this study, the secretory TK-cystatin gene, encoding a protein of 120 amino acid residues (13.65 kDa), was cloned from T. kitauei genome. Phylogenetic analysis showed that TK-cystatin gene is closely related to the cystatin-A from Hydra vulgaris. Multiple sequence alignment revealed that TK-cystatin had three conserved motifs: N-terminal G
Identifiants
pubmed: 32577834
doi: 10.1186/s13568-020-01052-0
pii: 10.1186/s13568-020-01052-0
pmc: PMC7311561
doi:
Types de publication
Journal Article
Langues
eng
Pagination
116Subventions
Organisme : National Natural Science Foundation of China
ID : 31672294
Organisme : Beijing earmarked fund for Modern Agro-industry Technology Research System
ID : SCGWZJ20201104-4
Organisme : Innovation Capability Support Program of Shaanxi
ID : 2018TD-021
Organisme : Se-enriched Special Science and Technology Project of China Institute of Se-enriched Industry
ID : 2018FXZX02-04
Références
EMBO J. 2001 Sep 3;20(17):4629-33
pubmed: 11532926
Parasitol Res. 2017 Mar;116(3):901-908
pubmed: 28066871
Immunology. 2013 Apr;138(4):370-81
pubmed: 23240853
Curr Biol. 2001 Mar 20;11(6):447-51
pubmed: 11301256
Front Genet. 2014 Jul 09;5:206
pubmed: 25071834
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Parasitol Today. 1987 Jul;3(7):220-2
pubmed: 15462961
Infect Immun. 2001 Dec;69(12):7380-6
pubmed: 11705911
Dev Comp Immunol. 2014 Apr;43(2):243-56
pubmed: 23994774
Chem Rev. 2002 Dec;102(12):4459-88
pubmed: 12475197
J Biol Chem. 2016 Sep 9;291(37):19220-34
pubmed: 27422822
Mol Phylogenet Evol. 2014 Dec;81:10-8
pubmed: 25192780
Nat Protoc. 2015 Jun;10(6):845-58
pubmed: 25950237
FEBS Lett. 1997 Apr 21;407(1):47-50
pubmed: 9141479
Syst Parasitol. 2006 Oct;65(2):97-114
pubmed: 16676228
Parasit Vectors. 2018 Mar 12;11(1):177
pubmed: 29530076
Eur J Immunol. 1997 Sep;27(9):2253-60
pubmed: 9341767
Mol Biol Evol. 1987 Jul;4(4):406-25
pubmed: 3447015
J Mol Biol. 2003 Feb 21;326(3):875-85
pubmed: 12581647
Nucleic Acids Res. 2013 Jan;41(Database issue):D344-7
pubmed: 23161676
Wei Sheng Wu Xue Bao. 2016 May 04;56(5):787-803
pubmed: 29727141
Parasite Immunol. 2017 Apr;39(4):
pubmed: 28295446
Semin Cell Dev Biol. 2000 Jun;11(3):203-10
pubmed: 10906277
Biochem J. 2010 Jul 1;429(1):103-12
pubmed: 20545626
Sci Rep. 2017 Dec 13;7(1):17526
pubmed: 29235483
J Immunol. 2001 Sep 15;167(6):3207-15
pubmed: 11544307
Nat Rev Immunol. 2003 Jun;3(6):472-82
pubmed: 12776207
Oncotarget. 2017 Jul 4;8(27):44108-44120
pubmed: 28484087
Evolution. 1985 Jul;39(4):783-791
pubmed: 28561359
J Immunol. 2009 Jun 15;182(12):7422-9
pubmed: 19494265
Genome Biol Evol. 2014 Nov 08;6(12):3182-98
pubmed: 25381665
Bioinformatics. 2014 Jun 15;30(12):1771-3
pubmed: 24532726
J Am Vet Med Assoc. 2011 Mar 15;238(6):784-6
pubmed: 21401436
Biochimie. 2012 May;94(5):1119-27
pubmed: 22285967
EMBO J. 2013 May 15;32(10):1478-88
pubmed: 23584533
Parasitol Int. 2014 Jun;63(3):489-91
pubmed: 24468917
Cell Mol Life Sci. 2002 Sep;59(9):1503-12
pubmed: 12440772
Infect Immun. 2003 May;71(5):2422-9
pubmed: 12704112
Parasitol Res. 2016 Nov;115(11):4317-4325
pubmed: 27492197