Development of a fluorine-18 radiolabelled fluorescent chalcone: evaluated for detecting glycogen.
Journal
EJNMMI radiopharmacy and chemistry
ISSN: 2365-421X
Titre abrégé: EJNMMI Radiopharm Chem
Pays: England
ID NLM: 101714628
Informations de publication
Date de publication:
23 Jun 2020
23 Jun 2020
Historique:
received:
05
03
2020
accepted:
02
06
2020
entrez:
25
6
2020
pubmed:
25
6
2020
medline:
25
6
2020
Statut:
epublish
Résumé
Glycogen is a multibranched polysaccharide of glucose produced by cells to store energy and plays a key role in cancer. A previously reported fluorescent probe (CDg4) was shown to selectively bind glycogen in mouse embryonic stem cells, however the molecule was not evaluated in cancer cells. We report the synthesis and biological evaluation of a dual-modality imaging probe based on CDg4, for positron emission tomography (PET) and fluorescence microscopy. A fluorine-18 radiolabelled derivative of CDg4, ([ Fluorescent compound 5 selectively accumulated in glycogen containing cancer cells, identified by fluorescence microscopy; however, rapid in vivo metabolic degradation precludes further investigation of [
Sections du résumé
BACKGROUND
BACKGROUND
Glycogen is a multibranched polysaccharide of glucose produced by cells to store energy and plays a key role in cancer. A previously reported fluorescent probe (CDg4) was shown to selectively bind glycogen in mouse embryonic stem cells, however the molecule was not evaluated in cancer cells. We report the synthesis and biological evaluation of a dual-modality imaging probe based on CDg4, for positron emission tomography (PET) and fluorescence microscopy.
RESULTS
RESULTS
A fluorine-18 radiolabelled derivative of CDg4, ([
CONCLUSIONS
CONCLUSIONS
Fluorescent compound 5 selectively accumulated in glycogen containing cancer cells, identified by fluorescence microscopy; however, rapid in vivo metabolic degradation precludes further investigation of [
Identifiants
pubmed: 32578021
doi: 10.1186/s41181-020-00098-6
pii: 10.1186/s41181-020-00098-6
pmc: PMC7311592
doi:
Types de publication
Journal Article
Langues
eng
Pagination
17Subventions
Organisme : Kings and Imperial College London EPSRC Centre for Doctoral Training in Medical Imaging
ID : EP/L015226/1
Organisme : Medical Research Council
ID : MR/N020782/1
Pays : United Kingdom
Organisme : NIHR Imperial Biomedical Research Centre
ID : WSCC_P62585
Organisme : Experimental Cancer Medicines Centres
ID : C37/A7283
Organisme : Cancer Research UK
ID : C2536/A16584
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC-A652-5PY80
Pays : United Kingdom
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