Modeling of Wnt-mediated tissue patterning in vertebrate embryogenesis.
Animals
Apoptosis
Body Patterning
/ physiology
Brain
/ embryology
Cell Lineage
Cell Movement
Computational Biology
Computer Simulation
Embryonic Development
Gene Expression Regulation, Developmental
Neural Crest
/ embryology
Neural Plate
/ embryology
Protein Transport
Signal Transduction
Software
Stochastic Processes
Vertebrates
/ embryology
Wnt Proteins
/ physiology
Zebrafish
/ embryology
beta Catenin
/ physiology
Journal
PLoS computational biology
ISSN: 1553-7358
Titre abrégé: PLoS Comput Biol
Pays: United States
ID NLM: 101238922
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
19
09
2019
accepted:
14
05
2020
revised:
07
07
2020
pubmed:
25
6
2020
medline:
25
8
2020
entrez:
25
6
2020
Statut:
epublish
Résumé
During embryogenesis, morphogens form a concentration gradient in responsive tissue, which is then translated into a spatial cellular pattern. The mechanisms by which morphogens spread through a tissue to establish such a morphogenetic field remain elusive. Here, we investigate by mutually complementary simulations and in vivo experiments how Wnt morphogen transport by cytonemes differs from typically assumed diffusion-based transport for patterning of highly dynamic tissue such as the neural plate in zebrafish. Stochasticity strongly influences fate acquisition at the single cell level and results in fluctuating boundaries between pattern regions. Stable patterning can be achieved by sorting through concentration dependent cell migration and apoptosis, independent of the morphogen transport mechanism. We show that Wnt transport by cytonemes achieves distinct Wnt thresholds for the brain primordia earlier compared with diffusion-based transport. We conclude that a cytoneme-mediated morphogen transport together with directed cell sorting is a potentially favored mechanism to establish morphogen gradients in rapidly expanding developmental systems.
Identifiants
pubmed: 32579554
doi: 10.1371/journal.pcbi.1007417
pii: PCOMPBIOL-D-19-01606
pmc: PMC7340325
doi:
Substances chimiques
Wnt Proteins
0
beta Catenin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1007417Subventions
Organisme : Medical Research Council
ID : MR/S007970/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT105618MA
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P01478X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S016295/1
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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