Histological Correlates of Diffusion-Weighted Magnetic Resonance Microscopy in a Mouse Model of Mesial Temporal Lobe Epilepsy.
HARDI
MR microscopy
hippocampus
kainate
mesial temporal lobe epilepsy
tractography
Journal
Frontiers in neuroscience
ISSN: 1662-4548
Titre abrégé: Front Neurosci
Pays: Switzerland
ID NLM: 101478481
Informations de publication
Date de publication:
2020
2020
Historique:
received:
09
03
2020
accepted:
04
05
2020
entrez:
26
6
2020
pubmed:
26
6
2020
medline:
26
6
2020
Statut:
epublish
Résumé
Mesial temporal lobe epilepsy (MTLE) is the most common type of focal epilepsy. It is frequently associated with abnormal MRI findings, which are caused by underlying cellular, structural, and chemical changes at the micro-scale. In the current study, it is investigated to which extent these alterations correspond to imaging features detected by high resolution magnetic resonance imaging in the intrahippocampal kainate mouse model of MTLE. Fixed hippocampal and whole-brain sections of mouse brain tissue from nine animals under physiological and chronically epileptic conditions were examined using structural and diffusion-weighted MRI. Microstructural details were investigated based on a direct comparison with immunohistochemical analyses of the same specimen. Within the hippocampal formation, diffusion streamlines could be visualized corresponding to dendrites of CA1 pyramidal cells and granule cells, as well as mossy fibers and Schaffer collaterals. Statistically significant changes in diffusivities, fractional anisotropy, and diffusion orientations could be detected in tissue samples from chronically epileptic animals compared to healthy controls, corresponding to microstructural alterations (degeneration of pyramidal cells, dispersion of the granule cell layer, and sprouting of mossy fibers). The diffusion parameters were significantly correlated with histologically determined cell densities. These findings demonstrate that high-resolution diffusion-weighted MRI can resolve subtle microstructural changes in epileptic hippocampal tissue corresponding to histopathological features in MTLE.
Identifiants
pubmed: 32581687
doi: 10.3389/fnins.2020.00543
pmc: PMC7284165
doi:
Types de publication
Journal Article
Langues
eng
Pagination
543Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2020 Göbel-Guéniot, Gerlach, Kamberger, Leupold, von Elverfeldt, Hennig, Korvink, Haas and LeVan.
Références
Magn Reson Med. 2001 Nov;46(5):946-54
pubmed: 11675647
Neuroimage. 2015 Sep;118:667-75
pubmed: 26072250
Brain Struct Funct. 2014 Mar;219(2):683-706
pubmed: 23474541
Neuroimage. 2010 Jun;51(2):521-30
pubmed: 20211740
Neuroimage. 2008 May 1;40(4):1718-30
pubmed: 18328732
Neuroscience. 1999 Mar;89(3):717-29
pubmed: 10199607
AJNR Am J Neuroradiol. 2003 Oct;24(9):1857-62
pubmed: 14561616
Proc Natl Acad Sci U S A. 2010 Jul 13;107(28):12687-91
pubmed: 20616040
J Neurosci. 2006 Apr 26;26(17):4701-13
pubmed: 16641251
Magn Reson Med. 2014 Oct;72(4):1151-61
pubmed: 24243402
Hippocampus. 2011 Mar;21(3):334-43
pubmed: 20865735
Cereb Cortex. 2012 Jan;22(1):26-36
pubmed: 21572089
Neuroimage. 2008 May 1;40(4):1619-32
pubmed: 18342541
Amyotroph Lateral Scler Frontotemporal Degener. 2019 Nov;20(7-8):549-561
pubmed: 31159586
Neuroimage. 2002 Apr;15(4):892-901
pubmed: 11906229
Magn Reson Med. 2008 Feb;59(2):268-77
pubmed: 18228601
Adv Exp Med Biol. 2012;724:99-113
pubmed: 22411237
Neuroscience. 1995 Feb;64(3):665-74
pubmed: 7715779
Neuroimage. 2011 Aug 15;57(4):1458-65
pubmed: 21575730
Neurobiol Dis. 2011 Apr;42(1):35-47
pubmed: 21220014
Neurophysiol Clin. 2010 Jun;40(3):151-7
pubmed: 20513614
Front Neuroanat. 2014 Jun 13;8:47
pubmed: 24982617
Neuroimage. 2016 Jan 15;125:84-93
pubmed: 26499812
Elife. 2017 Jul 26;6:
pubmed: 28746029
Brain Res. 2005 Dec 20;1066(1-2):129-46
pubmed: 16359649
Seizure. 2018 Nov;62:3-10
pubmed: 30245458
J Neuropathol Exp Neurol. 2002 Jun;61(6):510-9
pubmed: 12071634
NMR Biomed. 2016 Apr;29(4):458-65
pubmed: 26835664
Neuroscience. 2002;112(1):101-11
pubmed: 12044475
AJNR Am J Neuroradiol. 2015 Apr;36(4):719-24
pubmed: 25500311
Neuroimage. 2011 Jan 15;54(2):955-62
pubmed: 20854913
Neuroimage. 2004;23 Suppl 1:S208-19
pubmed: 15501092
AJNR Am J Neuroradiol. 2007 May;28(5):958-64
pubmed: 17494678
Epilepsia. 2013 Jul;54(7):1315-29
pubmed: 23692496
Front Cell Neurosci. 2013 Oct 01;7:167
pubmed: 24098270
Neuroimage. 2014 Nov 15;102 Pt 2:381-92
pubmed: 25087481
Neuroimage. 2010 Aug 15;52(2):556-61
pubmed: 20403443
Cereb Cortex. 2015 Nov;25(11):4628-37
pubmed: 26048951
Epilepsia. 2005 Apr;46(4):470-2
pubmed: 15816939
Epilepsy Res. 1996 Dec;26(1):141-50
pubmed: 8985696
NMR Biomed. 2003 Apr;16(2):77-81
pubmed: 12730948
Hippocampus. 2016 May;26(5):577-88
pubmed: 26482541
J Neurosci. 2002 Jul 15;22(14):5797-802
pubmed: 12122039
Neoplasia. 2014 Dec;16(12):993-1006
pubmed: 25499213
Epilepsia. 2005 Feb;46(2):193-202
pubmed: 15679500
Brain Struct Funct. 2015 Mar;220(2):781-801
pubmed: 24363120
Neuroimage. 2010 Jul 15;51(4):1360-6
pubmed: 20350604
Invest Radiol. 2017 Nov;52(11):666-671
pubmed: 28538339
Magn Reson Med. 2006 Oct;56(4):927-31
pubmed: 16941621
Neuroimage. 2012 Oct 15;63(1):1-10
pubmed: 22759994
Neuroimage. 2009 Jun;46(2):411-8
pubmed: 19233299
Neuroimage. 2006 Oct 1;32(4):1499-509
pubmed: 16806988
Neuron. 2000 Oct;28(1):41-51
pubmed: 11086982
Neuroimage Clin. 2013 Aug 17;3:196-201
pubmed: 24179863