Growth and Break-Up of Methanogenic Granules Suggests Mechanisms for Biofilm and Community Development.
anaerobic digestion
biofilms
methanogens
microbial communities
sludge granules
wastewater
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
28
01
2020
accepted:
05
05
2020
entrez:
26
6
2020
pubmed:
26
6
2020
medline:
26
6
2020
Statut:
epublish
Résumé
Methanogenic sludge granules are densely packed, small, spherical biofilms found in anaerobic digesters used to treat industrial wastewaters, where they underpin efficient organic waste conversion and biogas production. Each granule theoretically houses representative microorganisms from all of the trophic groups implicated in the successive and interdependent reactions of the anaerobic digestion (AD) process. Information on exactly how methanogenic granules develop, and their eventual fate will be important for precision management of environmental biotechnologies. Granules from a full-scale bioreactor were size-separated into small (0.6-1 mm), medium (1-1.4 mm), and large (1.4-1.8 mm) size fractions. Twelve laboratory-scale bioreactors were operated using either small, medium, or large granules, or unfractionated sludge. After >50 days of operation, the granule size distribution in each of the small, medium, and large bioreactor sets had diversified beyond-to both bigger and smaller than-the size fraction used for inoculation. Interestingly, extra-small (XS; <0.6 mm) granules were observed, and retained in all of the bioreactors, suggesting the continuous nature of granulation, and/or the breakage of larger granules into XS bits. Moreover, evidence suggested that even granules with small diameters could break. "New" granules from each emerging size were analyzed by studying community structure based on high-throughput 16S rRNA gene sequencing.
Identifiants
pubmed: 32582085
doi: 10.3389/fmicb.2020.01126
pmc: PMC7285868
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1126Subventions
Organisme : Medical Research Council
ID : MR/L015080/1
Pays : United Kingdom
Informations de copyright
Copyright © 2020 Trego, Galvin, Sweeney, Dunning, Murphy, Mills, Nzeteu, Quince, Connelly, Ijaz and Collins.
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