Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters.
biosynthetic gene cluster
direct cloning
heterologous expression
natural product
sequence-independent cloning
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
03
2020
accepted:
04
05
2020
entrez:
26
6
2020
pubmed:
26
6
2020
medline:
26
6
2020
Statut:
epublish
Résumé
Since the discovery of penicillin, natural products and their derivatives have been a valuable resource for drug discovery. With recent development of genome mining approaches in the post-genome era, a great number of natural product biosynthetic gene clusters (BGCs) have been identified and these can potentially be exploited for the discovery of novel natural products that can find application as pharmaceuticals. Since many BGCs are silent or do not express in native hosts under laboratory conditions, heterologous expression of BGCs in genetically tractable hosts becomes an attractive route to activate these BGCs to discover the corresponding products. Here, we highlight recent achievements in cloning and discovery of natural product biosynthetic pathways via intact BGC capturing, and discuss the prospects of high-throughput and multiplexed cloning of rational-designed gene clusters in the future.
Identifiants
pubmed: 32582659
doi: 10.3389/fbioe.2020.00526
pmc: PMC7290108
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
526Informations de copyright
Copyright © 2020 Lin, Nielsen and Liu.
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