A Role for Taok2 in Listeria monocytogenes Vacuolar Escape.
Listeria monocytogenes
STE1-like kinase
Taok2
siRNA screen
vacuolar escape
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
15 03 2022
15 03 2022
Historique:
received:
18
04
2020
accepted:
19
06
2020
pubmed:
26
6
2020
medline:
10
5
2022
entrez:
26
6
2020
Statut:
ppublish
Résumé
The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.
Identifiants
pubmed: 32582947
pii: 5862450
doi: 10.1093/infdis/jiaa367
pmc: PMC8922001
doi:
Substances chimiques
Bacterial Proteins
0
Hemolysin Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1005-1010Informations de copyright
© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.
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