Human apolipoprotein L1 interferes with mitochondrial function in Saccharomyces cerevisiae.
apolipoprotein l1
yeast
mitochondrion
respiratory index
fermentation
Journal
Molecular medicine reports
ISSN: 1791-3004
Titre abrégé: Mol Med Rep
Pays: Greece
ID NLM: 101475259
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
20
10
2019
accepted:
24
04
2020
pubmed:
26
6
2020
medline:
21
4
2021
entrez:
26
6
2020
Statut:
ppublish
Résumé
To the best of our knowledge, the vertebrate apolipoprotein L (APOL) family has not previously been ascribed to any definite pathophysiological function, although the conserved BH3 protein domain suggests a role in programmed cell death or an interference with mitochondrial processes. In the present study, the human APOL1 was expressed in the yeast Saccharomyces cerevisiae in order to determine the molecular action of APOL1. APOL1 inhibited cell proliferation in a non‑fermentable carbon source, such as glycerol, while it had no effect on proliferation in fermentable carbon sources, such as galactose. APOL1, expressed in yeast, is localized in the mitochondrial fraction, as determined via western blotting. APOL1 induced a loss of mitochondrial function, demonstrated by a loss of respiratory index, and mitochondrial membrane potential. Green fluorescent protein tagging of mitochondrial protein revealed that APOL1 was associated with abnormal mitochondrial and lysosomal morphologies, observed by a loss of the normal mitochondrial tubular network. Thus, the results of the present study suggest that APOL1 could be a physiological regulator of mitochondrial function.
Identifiants
pubmed: 32583004
doi: 10.3892/mmr.2020.11271
pmc: PMC7411449
doi:
Substances chimiques
APOL1 protein, human
0
Apolipoprotein L1
0
Recombinant Proteins
0
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1910-1920Références
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