Developmental stages of zebrafish (Danio rerio) embryos and toxicological studies using foldscope microscope.
aromatic turmerone
developmental stages
foldscope
toxicology
zebrafish
Journal
Cell biology international
ISSN: 1095-8355
Titre abrégé: Cell Biol Int
Pays: England
ID NLM: 9307129
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
13
03
2020
revised:
17
06
2020
accepted:
23
06
2020
pubmed:
26
6
2020
medline:
11
8
2021
entrez:
26
6
2020
Statut:
ppublish
Résumé
Zebrafish (Danio rerio), is a well-established vertebrate animal model widely used in developmental biology and toxicological research. In the present study, foldscope is used as an innovative tool to study the developmental stages and toxicological analysis of the zebrafish embryos. Briefly, the developmental stages, such as zygote, cleavage, blastula, gastrula, segmentation, and pharyngula formation are observed and documented using simple foldscope. Toxicological parameters upon exposure to different concentration of ethanol extract of Curcuma longa and its lead compound, ar-turmerone along with rhodamine B (bio-coupler) on zebrafish embryos are analyzed upto 72 hr using foldscopes in live condition. The lethal endpoints, such as coagulation, lack of somite formation, non-detachment of tail, and lack of heartbeat are clearly monitored and documented using foldscope. Bio-evaluation of test compounds with the aid of foldscope confirms that the toxicity is directly proportional to the concentration. Our results conclude that, ethanol extract of C. longa, ar-turmerone and rhodamine B exposed embryos remains healthy up to 96, 48, and 24 µg concentrations, respectively. Embryos exposed to higher concentrations become coagulated, however normal physiological active movement of tail lashing and heartbeat are evident in lower concentration exposed embryos. Except coagulation, no other abnormalities are observed and interestingly, the hatching ability is not delayed, when compared with the control embryos. It is confirmed that the test compounds are not highly toxic to zebrafish embryos. Hence it can be used for further analysis, especially for studying the neural-regeneration and its neuronal development in zebrafish embryos.
Substances chimiques
Plant Extracts
0
Rhodamines
0
rhodamine B
K7G5SCF8IL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1968-1980Subventions
Organisme : Indian Council of Medical Research
ID : 2017-0898/SCRR-BMS
Organisme : Department of Biotechnology, Ministry of Science and Technology
ID : BT/IN/Indo-US/Foldscopes/39/2015
Informations de copyright
© 2020 International Federation for Cell Biology.
Références
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