Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
24
02
2020
accepted:
30
04
2020
revised:
08
07
2020
pubmed:
26
6
2020
medline:
11
8
2020
entrez:
26
6
2020
Statut:
epublish
Résumé
The human adenovirus type 5 (HAdV5) causes disease of the upper and lower respiratory tract. The early steps of HAdV5 entry up to genome replication in the host nucleus have been extensively studied. However, late stages of infection remain poorly understood. Here, we set out to elucidate the spatiotemporal orchestration of late adenovirus nuclear remodeling in living cells. We generated virus mutants expressing fluorescently tagged protein IX (pIX) and protein V (pV), a capsid and viral genome associated protein, respectively. We found that during progeny virion production both proteins localize to a membrane-less, nuclear compartment, which is highly impermeable such that in immunofluorescence microscopy antibodies can hardly penetrate it. We termed this compartment 'late virion accumulation compartment' (LVAC). Correlation between light- and electron microscopy revealed that the LVAC contains paracrystalline arrays of viral capsids that arrange tightly packed within a honeycomb-like organization of viral DNA. Live-cell microscopy as well as FRAP measurements showed that the LVAC is rigid and restricts diffusion of larger molecules, indicating that capsids are trapped inside.
Identifiants
pubmed: 32584886
doi: 10.1371/journal.ppat.1008588
pii: PPATHOGENS-D-20-00364
pmc: PMC7343190
doi:
Substances chimiques
Capsid Proteins
0
DNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008588Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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