Protein phosphatase 1 alpha enhances glucocorticoid receptor activity by a mechanism involving phosphorylation of serine-211.

A549 Cells Binding Sites Gene Knockdown Techniques HEK293 Cells Humans Phosphorylation / genetics Protein Interaction Domains and Motifs / genetics Protein Phosphatase 1 / genetics Protein Processing, Post-Translational / genetics Receptors, Glucocorticoid / chemistry Serine / genetics
Glucocorticoid receptor Glycogen synthase kinase 3 Phosphorylation Protein phosphatase 1 alpha Signaling

Journal

Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844

Informations de publication

Date de publication:
01 12 2020
Historique:
received: 22 11 2019
revised: 17 04 2020
accepted: 18 05 2020
pubmed: 26 6 2020
medline: 29 6 2021
entrez: 26 6 2020
Statut: ppublish

Résumé

By acting as a ligand-dependent transcription factor the glucocorticoid receptor (GR) mediates the actions of glucocorticoids and regulates many physiological processes. An impaired regulation of glucocorticoid action has been associated with numerous disorders. Thus, the elucidation of underlying signaling pathways is essential to understand mechanisms of disrupted glucocorticoid function and contribution to diseases. This study found increased GR transcriptional activity upon overexpression of protein phosphatase 1 alpha (PP1α) in HEK-293 cells and decreased expression levels of GR-responsive genes following PP1α knockdown in the endogenous A549 cell model. Mechanistic investigations revealed reduced phosphorylation of GR-Ser211 following PP1α silencing and provided a first indication for an involvement of glycogen synthase kinase 3 (GSK-3). Thus, the present study identified PP1α as a novel post-translational activator of GR signaling, suggesting that disruption of PP1α function could lead to impaired glucocorticoid action and thereby contribute to diseases.

Identifiants

DOI: 10.1016/j.mce.2020.110873 PMID: 32585168 PMC: PMC7606615
pubmed: 32585168
pii: S0303-7207(20)30173-8
doi: 10.1016/j.mce.2020.110873
pmc: PMC7606615
mid: NIHMS1627932
pii:
doi:

Substances chimiques

Receptors, Glucocorticoid 0
Serine 452VLY9402
Protein Phosphatase 1 EC 3.1.3.16

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

110873

Subventions

Organisme : Intramural NIH HHS
ID : Z01 ES090057
Pays : United States

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Références

Nat Cell Biol. 2001 Jan;3(1):93-6
pubmed: 11146632
Mol Cell Biol. 1997 Jul;17(7):3947-54
pubmed: 9199329
Genes Dev. 2001 Sep 15;15(18):2367-80
pubmed: 11562347
BMC Mol Biol. 2004 Sep 06;5:15
pubmed: 15350195
J Biol Chem. 2017 Apr 7;292(14):5825-5844
pubmed: 28223352
Sci Rep. 2017 Aug 21;7(1):8941
pubmed: 28827617
Curr Cancer Drug Targets. 2014;14(1):2-29
pubmed: 24200083
J Biol Chem. 2003 Aug 29;278(35):33067-77
pubmed: 12796505
Brain Res Mol Brain Res. 2004 Apr 7;123(1-2):27-36
pubmed: 15046863
Pathol Oncol Res. 2016 Apr;22(2):287-92
pubmed: 26253838
EMBO J. 1990 Aug;9(8):2431-8
pubmed: 2164470
Mol Cell Biol. 2008 Dec;28(24):7309-22
pubmed: 18838540
Chem Rev. 2001 Aug;101(8):2527-40
pubmed: 11749387
J Cell Sci. 2002 Jan 15;115(Pt 2):241-56
pubmed: 11839776
Methods Enzymol. 1991;200:564-77
pubmed: 1659660
Nature. 2002 Aug 29;418(6901):970-5
pubmed: 12198546
Mol Endocrinol. 1990 Mar;4(3):455-64
pubmed: 2160602
Nat Rev Mol Cell Biol. 2007 Mar;8(3):234-44
pubmed: 17318227
IUBMB Life. 2009 Oct;61(10):979-86
pubmed: 19787703
Mol Cell Biochem. 1998 May;182(1-2):49-58
pubmed: 9609113
Eur J Biochem. 1980 Jun;107(2):519-27
pubmed: 6249596
J Alzheimers Dis. 2008 Oct;15(2):181-91
pubmed: 18953107
F1000Res. 2017 Jul 24;6:1208
pubmed: 28781762
Front Immunol. 2019 Oct 17;10:2460
pubmed: 31681333
Prog Nucleic Acid Res Mol Biol. 2003;75:173-216
pubmed: 14604013
J Steroid Biochem Mol Biol. 1995 Nov;55(2):135-46
pubmed: 7495692
FEBS Lett. 1989 Jul 3;250(2):596-600
pubmed: 2546812
Biomed Pharmacother. 2006 Nov;60(9):520-8
pubmed: 16949786
Mol Cell Biol. 2010 Jan;30(1):220-30
pubmed: 19841061
Mol Endocrinol. 1991 Sep;5(9):1215-28
pubmed: 1663212
J Biol Chem. 2003 Jun 20;278(25):22748-54
pubmed: 12686538
J Biol Chem. 1997 Apr 4;272(14):9287-93
pubmed: 9083064
Mol Cell Endocrinol. 2017 Jan 5;439:431-443
pubmed: 27717743
Mol Endocrinol. 2005 Jun;19(6):1474-82
pubmed: 15761032
Trends Endocrinol Metab. 2008 Nov;19(9):331-9
pubmed: 18805703
J Biol Chem. 2005 Nov 11;280(45):37526-35
pubmed: 16155008
FEBS Lett. 1994 Jan 24;338(1):37-42
pubmed: 8307153
Mol Cell Biochem. 2007 Nov;305(1-2):45-54
pubmed: 17646931
Methods. 2010 Apr;50(4):S1-5
pubmed: 20215014
Mol Cell Endocrinol. 2017 Jul 15;450:74-82
pubmed: 28454724
Physiol Rev. 2004 Jan;84(1):1-39
pubmed: 14715909
Biochem J. 1993 Nov 15;296 ( Pt 1):15-9
pubmed: 8250835
Am J Respir Cell Mol Biol. 2012 Oct;47(4):464-73
pubmed: 22592921
Ann N Y Acad Sci. 2004 Jun;1024:86-101
pubmed: 15265775
J Steroid Biochem Mol Biol. 2010 May 31;120(2-3):76-85
pubmed: 20188830
PLoS One. 2011;6(12):e27627
pubmed: 22205926
Mol Endocrinol. 1992 Jan;6(1):26-34
pubmed: 1310797
Cell Mol Life Sci. 2007 Aug;64(15):1930-44
pubmed: 17530463
J Biol Chem. 2001 Nov 16;276(46):42714-21
pubmed: 11555652
J Biol Chem. 1998 Jun 5;273(23):14315-21
pubmed: 9603939
Arch Biochem Biophys. 2008 Jul 1;475(1):1-6
pubmed: 18424253
J Neurochem. 2010 Nov;115(4):974-83
pubmed: 20831597
Oncotarget. 2016 Jan 12;7(2):1754-64
pubmed: 26636645
Curr Drug Targets. 2006 Nov;7(11):1435-41
pubmed: 17100583
EMBO J. 2004 Jun 2;23(11):2235-45
pubmed: 15152189
Steroids. 2005 May-Jun;70(5-7):407-17
pubmed: 15862824
Mol Endocrinol. 2012 Sep;26(9):1508-20
pubmed: 22771494
Mol Endocrinol. 2010 Jun;24(6):1136-50
pubmed: 20371704
Steroids. 2007 Jun;72(6-7):600-8
pubmed: 17509631
J Biol Chem. 2019 Jul 19;294(29):11225-11239
pubmed: 31167788
Protein Sci. 2006 Apr;15(4):722-30
pubmed: 16600964
Cancer Cell Int. 2007 Mar 28;7:3
pubmed: 17391526
Steroids. 2012 Sep;77(11):1041-9
pubmed: 22728894
Mol Endocrinol. 2005 Jun;19(6):1569-83
pubmed: 15817653
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Mol Endocrinol. 2007 Jul;21(7):1552-68
pubmed: 17440046
Genes Brain Behav. 2008 Feb;7 Suppl 1:57-66
pubmed: 18184370
Cell. 2001 Jun 29;105(7):821-4
pubmed: 11439177
Mol Endocrinol. 2002 Oct;16(10):2382-92
pubmed: 12351702
Mol Endocrinol. 2008 Aug;22(8):1754-66
pubmed: 18483179
Endocrinology. 2002 Sep;143(9):3482-9
pubmed: 12193561
Biochim Biophys Acta. 2008 Aug;1783(8):1536-43
pubmed: 18381077
J Biol Chem. 2002 Jul 19;277(29):26573-80
pubmed: 12000743
Diabetes. 1998 Sep;47(9):1519-24
pubmed: 9726244
J Steroid Biochem Mol Biol. 2016 May;159:31-40
pubmed: 26907965
J Steroid Biochem Mol Biol. 2010 Oct;122(1-3):10-20
pubmed: 20170729
Biochem J. 1988 Nov 15;256(1):283-90
pubmed: 2851982
Nat Rev Endocrinol. 2013 Nov;9(11):670-86
pubmed: 24080732
Endocrinology. 2011 Aug;152(8):3123-34
pubmed: 21558312
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2050-5
pubmed: 9482836
Cancer Res. 2010 Aug 15;70(16):6437-47
pubmed: 20663907
Br J Pharmacol. 2006 Jan;147 Suppl 1:S258-68
pubmed: 16402112
Mol Cell Biol. 2011 Dec;31(23):4663-75
pubmed: 21930780
Mol Cell Biol. 2003 Aug;23(16):5867-81
pubmed: 12897156
J Immunol. 2016 Nov 15;197(10):3782-3791
pubmed: 27742828

Auteurs

Melanie Patt (M)

Swiss Centre for Applied Human Toxicology (SCAHT), University of Basel, Missionsstrasse 64, 4055, Basel, Switzerland; Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland. Electronic address: melanie.patt@unibas.ch.

Joël Gysi (J)

Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland. Electronic address: joel.gysi@gmx.ch.

Nourdine Faresse (N)

DIVA Expertise, 31100, Toulouse, France. Electronic address: nourdine.faresse@diva-expertise.com.

John A Cidlowski (JA)

Signal Transduction Laboratory, NIEHS, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC, 27709, USA. Electronic address: cidlows1@niehs.nih.gov.

Alex Odermatt (A)

Swiss Centre for Applied Human Toxicology (SCAHT), University of Basel, Missionsstrasse 64, 4055, Basel, Switzerland; Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland. Electronic address: Alex.Odermatt@unibas.ch.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules A549 › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-down de gènes › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Métabolisme › Phosphorylation › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Motifs et domaines d'intéraction protéique › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphoprotein Phosphatases › Protein Phosphatase 1 › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Récepteurs cytoplasmiques et nucléaires › Récepteurs aux glucocorticoïdes › Protein phosphatase 1 alpha enhances...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Sérine › Protein phosphatase 1 alpha enhances...