Development of a cell-free split-luciferase biochemical assay as a tool for screening for inhibitors of challenging protein-protein interaction targets.
KRAS
PI3K
RAF
luciferase
protein-protein interaction
screening
Journal
Wellcome open research
ISSN: 2398-502X
Titre abrégé: Wellcome Open Res
Pays: England
ID NLM: 101696457
Informations de publication
Date de publication:
2020
2020
Historique:
accepted:
29
01
2020
entrez:
27
6
2020
pubmed:
27
6
2020
medline:
27
6
2020
Statut:
epublish
Résumé
Targeting the interaction of proteins with weak binding affinities or low solubility represents a particular challenge for drug screening. The NanoLuc
Identifiants
pubmed: 32587898
doi: 10.12688/wellcomeopenres.15675.1
pmc: PMC7308888
doi:
Types de publication
Journal Article
Langues
eng
Pagination
20Subventions
Organisme : Wellcome Trust
ID : 103799
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC001070
Pays : United Kingdom
Organisme : Medical Research Council
ID : FC001070
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001070
Pays : United Kingdom
Informations de copyright
Copyright: © 2020 Cooley R et al.
Déclaration de conflit d'intérêts
Competing interests: Competing interests: B.F.B and K.V.W. are employees of Promega Corporation and J.T. is an employee of AstraZeneca. J.D. has acted as a consultant for AstraZeneca, Bayer, Novartis, Vividion and TheRas. None of the other authors of this manuscript have a financial interest related to this work.
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