Attempts at the Development of a Recombinant African Swine Fever Virus Strain with Abrogated
9GL
A238L
African swine fever
CRISPR/Cas9
EP402R
Journal
Journal of veterinary research
ISSN: 2450-7393
Titre abrégé: J Vet Res
Pays: Poland
ID NLM: 101696630
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
04
10
2019
accepted:
25
05
2020
entrez:
27
6
2020
pubmed:
27
6
2020
medline:
27
6
2020
Statut:
epublish
Résumé
African swine fever (ASF) is a pressing economic problem in a number of Eastern European countries. It has also depleted the Chinese sow population by 50%. Managing the disease relies on culling infected pigs or hunting wild boars as sanitary zone creation. The constraints on the development of an efficient vaccine are mainly the virus' mechanisms of host immune response evasion. The study aimed to adapt a field ASFV strain to established cell lines and to construct recombinant African swine fever virus (ASFV) strain. The host immune response modulation genes The reconstituted virus showed similar kinetics of replication in comparison to the parent virus isolate. Taking into account the usefulness of the developed CRISPR/Cas9 system it has been shown that modification of the
Identifiants
pubmed: 32587905
doi: 10.2478/jvetres-2020-0039
pii: jvetres-2020-0039
pmc: PMC7305649
doi:
Types de publication
Journal Article
Langues
eng
Pagination
197-205Informations de copyright
© 2020 G. Woźniakowski et al. published by Sciendo.
Déclaration de conflit d'intérêts
Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.
Références
Virus Res. 2013 Apr;173(1):168-79
pubmed: 23041357
J Virol. 1998 Apr;72(4):2881-9
pubmed: 9525608
Virus Res. 2013 Apr;173(1):110-21
pubmed: 23201582
Antiviral Res. 2019 May;165:34-41
pubmed: 30836106
Molecules. 2019 Apr 05;24(7):
pubmed: 30959782
J Virol. 2017 Oct 13;91(21):
pubmed: 28814514
Vet Immunol Immunopathol. 2004 Aug;100(3-4):117-34
pubmed: 15207450
Sci Rep. 2018 Feb 16;8(1):3154
pubmed: 29453406
Virology. 2004 Feb 20;319(2):337-42
pubmed: 14980493
Sci Rep. 2017 Sep 4;7(1):10369
pubmed: 28871180
Sci Rep. 2018 Jan 23;8(1):1449
pubmed: 29362418
Arch Virol. 2016 Jan;161(1):189-95
pubmed: 26497350
Transbound Emerg Dis. 2013 Feb;60(1):48-58
pubmed: 22394449
Front Vet Sci. 2018 Apr 16;5:77
pubmed: 29713637
J Virol. 2000 Feb;74(3):1275-85
pubmed: 10627538
Sci Rep. 2019 Mar 14;9(1):4556
pubmed: 30872594
Pol J Vet Sci. 2014;17(4):665-72
pubmed: 25638980
Virology. 2012 Nov 10;433(1):142-8
pubmed: 22902236
Virology. 1998 Apr 10;243(2):461-71
pubmed: 9568043
RNA Biol. 2019 Apr;16(4):380-389
pubmed: 30856357
J Virol. 2017 Feb 28;91(6):
pubmed: 28031363
J Virol. 2015 Jun;89(11):6048-56
pubmed: 25810553
Front Vet Sci. 2019 Apr 26;6:137
pubmed: 31106218
Transbound Emerg Dis. 2019 May;66(3):1399-1404
pubmed: 30667598
J Virol. 2015 Aug;89(16):8556-66
pubmed: 26063424
J Virol. 2015 Feb;89(4):2324-32
pubmed: 25505073
J Vet Res. 2019 Mar 22;63(1):17-25
pubmed: 30989131
Virus Res. 2019 May;265:150-155
pubmed: 30922809
J Gen Virol. 2005 Sep;86(Pt 9):2445-2450
pubmed: 16099902
Emerg Infect Dis. 2016 Jul;22(7):1201-7
pubmed: 27314611
J Virol. 2016 Dec 16;91(1):
pubmed: 27795430
Sci Rep. 2018 Apr 25;8(1):6510
pubmed: 29695831