Infrared attenuated total reflection spectroscopic surface analysis of bovine-tail intervertebral discs after UV-light-activated riboflavin-induced collagen cross-linking.
IR-ATR
IVD
UVA-CXL
attenuated total reflection
collagen cross-linking
infrared spectroscopy
intervertebral disc
riboflavin-induced cross-linking
spinal surgery
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
27
03
2020
revised:
31
05
2020
accepted:
10
06
2020
pubmed:
27
6
2020
medline:
24
6
2021
entrez:
27
6
2020
Statut:
ppublish
Résumé
The tensile strength of the intervertebral disc (IVD) is mainly maintained by collagen cross-links. Loss of collagen cross-linking combined with other age-related degenerative processes contributes to tissue weakening, biomechanical failure, disc herniation and pain. Exogenous collagen cross-linking has been identified as an effective therapeutic approach for restoring IVD tensile strength. The current state-of-the-art method to assess the extent of collagen cross-linking in tissues requires destructive procedures and high-performance liquid chromatography. In this study, we investigated the utility of infrared attenuated total reflection (IR-ATR) spectroscopy as a nondestructive analytical strategy to rapidly evaluate the extent of UV-light-activated riboflavin (B2)-induced collagen cross-linking in bovine IVD samples. Thirty-five fresh bovine-tail IVD samples were equally divided into five treatment groups: (a) untreated, (b) cell culture medium Dulbecco's Modified Eagle's Medium only, (c) B2 only, (d) UV-light only and (e) UV-light-B2. A total of 674 measurements have been acquired, and were analyzed via partial least squares discriminant analysis. This classification scheme unambiguously identified individual classes with a sensitivity >91% and specificity >92%. The obtained results demonstrate that IR-ATR spectroscopy reliably differentiates between different treatment categories, and promises an excellent tool for potential in vivo, nondestructive and real-time assessment of exogenous IVD cross-linking.
Identifiants
pubmed: 32589779
doi: 10.1002/jbio.202000110
doi:
Substances chimiques
Cross-Linking Reagents
0
Photosensitizing Agents
0
Collagen
9007-34-5
Riboflavin
TLM2976OFR
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000110Informations de copyright
© 2020 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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