Phase 1 clinical study of cell therapy with effective-mononuclear cells (E-MNC) for radiogenic xerostomia (first-in-human study) (FIH study on E-MNC therapy for radiogenic xerostomia).


Journal

Medicine
ISSN: 1536-5964
Titre abrégé: Medicine (Baltimore)
Pays: United States
ID NLM: 2985248R

Informations de publication

Date de publication:
26 Jun 2020
Historique:
entrez: 27 6 2020
pubmed: 27 6 2020
medline: 9 7 2020
Statut: ppublish

Résumé

Treatment for most patients with head and neck cancers includes ionizing radiation with or without chemotherapy. This treatment causes irreversible damage to salivary glands in the irradiation field accompanied by a loss of fluid-secreting acinar cells and a considerable decrease of saliva secretion. There is currently no adequate conventional treatment for this condition. In recent years, we developed an effective culture method to enhance the anti-inflammatory and vasculogenic phenotypes of peripheral blood mononuclear cells (PBMNCs), and such effectively conditioned PBMNC (E-MNC) therapy has shown promising improvements to the function of radiation-injured salivary glands in preclinical studies. However, the safety and effect of E-NMC therapy have yet assessed in human. The objective of this ongoing first-in-man study is to assess the safety, tolerability, and in part the efficacy of E-MNC therapy for treating radiation-induced xerostomia. This phase 1 first-in-man study is an open-label, single-center, two-step dose escalation study. A total of 6 patients, who had no recurrence of head and neck cancer over 5 years following radiation therapy and suffered from radiation-induced xerostomia, will receive a transplantation of E-NMCs derived from autologous PBMNCs to a submandibular gland. The duration of the intervention will be 1 year. To analyze the recovery of salivary secretion, a gum test will be performed. To analyze the recovery of atrophic salivary glands, computed tomography (CT), and magnetic resonance imaging (MRI) of salivary glands will be conducted. The primary endpoint is the safety of the protocol. The secondary endpoints are the changes from baseline in whole saliva secretion and salivary gland atrophy. This will be the first clinical study of regenerative therapy using E-MNCs for patients with severe radiation-induced xerostomia. The results of this study are expected to contribute to developing the low-invasive cell-based therapy for radiation-induced xerostomia. This study was registered with the Japan Registry of Clinical Trials (http://jrct.niph.go.jp) as jRCTb070190057.

Sections du résumé

BACKGROUND BACKGROUND
Treatment for most patients with head and neck cancers includes ionizing radiation with or without chemotherapy. This treatment causes irreversible damage to salivary glands in the irradiation field accompanied by a loss of fluid-secreting acinar cells and a considerable decrease of saliva secretion. There is currently no adequate conventional treatment for this condition. In recent years, we developed an effective culture method to enhance the anti-inflammatory and vasculogenic phenotypes of peripheral blood mononuclear cells (PBMNCs), and such effectively conditioned PBMNC (E-MNC) therapy has shown promising improvements to the function of radiation-injured salivary glands in preclinical studies. However, the safety and effect of E-NMC therapy have yet assessed in human. The objective of this ongoing first-in-man study is to assess the safety, tolerability, and in part the efficacy of E-MNC therapy for treating radiation-induced xerostomia.
METHODS/DESIGN METHODS
This phase 1 first-in-man study is an open-label, single-center, two-step dose escalation study. A total of 6 patients, who had no recurrence of head and neck cancer over 5 years following radiation therapy and suffered from radiation-induced xerostomia, will receive a transplantation of E-NMCs derived from autologous PBMNCs to a submandibular gland. The duration of the intervention will be 1 year. To analyze the recovery of salivary secretion, a gum test will be performed. To analyze the recovery of atrophic salivary glands, computed tomography (CT), and magnetic resonance imaging (MRI) of salivary glands will be conducted. The primary endpoint is the safety of the protocol. The secondary endpoints are the changes from baseline in whole saliva secretion and salivary gland atrophy.
DISCUSSION CONCLUSIONS
This will be the first clinical study of regenerative therapy using E-MNCs for patients with severe radiation-induced xerostomia. The results of this study are expected to contribute to developing the low-invasive cell-based therapy for radiation-induced xerostomia.
TRIAL REGISTRATION BACKGROUND
This study was registered with the Japan Registry of Clinical Trials (http://jrct.niph.go.jp) as jRCTb070190057.

Identifiants

pubmed: 32590759
doi: 10.1097/MD.0000000000020788
pii: 00005792-202006260-00034
pmc: PMC7328916
doi:

Types de publication

Clinical Trial Protocol Clinical Trial, Phase I Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e20788

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Auteurs

Yoshinori Sumita (Y)

Basic and Translational Research Center for Hard Tissue Disease.

Naoki Iwamoto (N)

Department of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki.

Makoto Seki (M)

CellAxia Inc., Tokyo.

Takako Yoshida (T)

Basic and Translational Research Center for Hard Tissue Disease.

Ryo Honma (R)

Basic and Translational Research Center for Hard Tissue Disease.
Department of Regenerative Oral Surgery, Unit of Translational Medicine.

Mayumi Iwatake (M)

Basic and Translational Research Center for Hard Tissue Disease.

Seigo Ohba (S)

Department of Regenerative Oral Surgery, Unit of Translational Medicine.

I Takashi (I)

Department of Regenerative Oral Surgery, Unit of Translational Medicine.

Yuka Hotokezaka (Y)

Department of Radiology and Cancer Biology, Nagasaki University Graduate School of Biomedical Sciences.

Hiroshi Harada (H)

Transfusion and Cell Therapy Unit, Nagasaki University.

Shinichiro Kuroshima (S)

Department of Applied Prosthodontics, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki.

Kazuhiro Nagai (K)

Transfusion and Cell Therapy Unit, Nagasaki University.

Takayuki Asahara (T)

Department of Regenerative Medicine Science, Tokai University School of Medicine, Isehara, Japan.

I Atsushi Kawakam (I)

Department of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki.

Izumi Asahina (I)

Department of Regenerative Oral Surgery, Unit of Translational Medicine.

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Classifications MeSH