Demonstrating the Manufacture of Human CAR-T Cells in an Automated Stirred-Tank Bioreactor.
CAR-T cells
bioprocessing
immunotherapy
manufacture
stirred-tank bioreactor
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
15
04
2020
revised:
01
06
2020
pubmed:
28
6
2020
medline:
16
3
2021
entrez:
28
6
2020
Statut:
ppublish
Résumé
Chimeric antigen receptor T-cell (CAR-T) therapies have proven clinical efficacy for the treatment of hematological malignancies. However, CAR-T cell therapies are prohibitively expensive to manufacture. The authors demonstrate the manufacture of human CAR-T cells from multiple donors in an automated stirred-tank bioreactor. The authors successfully produced functional human CAR-T cells from multiple donors under dynamic conditions in a stirred-tank bioreactor, resulting in overall cell yields which were significantly better than in static T-flask culture. At agitation speeds of 200 rpm and greater (up to 500 rpm), the CAR-T cells are able to proliferate effectively, reaching viable cell densities of >5 × 10
Identifiants
pubmed: 32592336
doi: 10.1002/biot.202000177
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000177Informations de copyright
© 2020 The Authors. Biotechnology Journal published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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