Electric field-induced changes in biomechanical properties in human dermal fibroblasts and a human skin equivalent.
F-actin
atomic force microscopy
cellular elasticity
electric stimulation
human skin equivalent
Journal
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
ISSN: 1600-0846
Titre abrégé: Skin Res Technol
Pays: England
ID NLM: 9504453
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
12
03
2020
accepted:
30
05
2020
pubmed:
1
7
2020
medline:
14
8
2021
entrez:
29
6
2020
Statut:
ppublish
Résumé
An electric field (EF) can be used to change the mechanical properties of cells and skin tissues. We demonstrate EF-induced elasticity changes in human dermal fibroblasts (HDFs) and a human skin equivalent and identify the underlying principles related to the changes. HDFs and human skin equivalent were stimulated with electric fields of 1.0 V/cm. Change in cellular elasticity was determined by using atomic force microscopy. Effects of EF on the biomechanical and chemical properties of a human skin equivalent were analyzed. In cells and tissues, the effects of EF on biomarkers of cellular elasticity were investigated at the gene and protein levels. In HDFs, the cellular elasticity was increased and the expression of biomarkers of cellular elasticity was regulated by the EF. Expression of the collagen protein in the human skin equivalent was changed by EF stimulation; however, changes in density and microstructure of the collagen fibrils were not significant. The viscoelasticity of the human skin equivalent increased in response to EF stimulation, but molecular changes were not observed in collagen. Elasticity of cells and human skin equivalent can be regulated by electrical stimulation. Especially, the change in cellular elasticity was dependent on cell age.
Substances chimiques
Biomarkers
0
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
914-922Subventions
Organisme : Ministry of Trade and Industry & Energy
ID : 20002725
Organisme : Kyung Hee University
ID : KHU-20181046
Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Références
Pullar CE. The Physiology of Bioelectricity in Development, Tissue Regeneration and Cancer. Boca Raton, FL: CRC Press; 2011:342.
Markx GH. The use of electric fields in tissue engineering. Organogenesis. 2008;4:11-17.
Meng S, Rouabhia M, Zhang Z. Electrical stimulation modulates osteoblast proliferation and bone protein production through heparin-bioactivated conductive scaffolds. Bioelectromagnetics. 2014;34:189-199.
Robinson KR, Messerli MA. The role of endogenous electric fields as directional signals in development, repair and invasion. BioEssays. 2003;25:759-766.
Mycielska ME, Djamgoz MB. Cellular mechanisms of direct-current electric field effects: galvanotaxis and metastatic disease. J Cell Sci. 2004;117:631-639.
McKasson MJ, Huang L, Robinson KR. Chick embryonic Schwann cells migrate anodally in small electrical fields. Exp Neurol. 2008;211:585-587.
Rajnicek AM, Robinson KR, McCaig CD. The direction of neurite growth in a weak DC electric field depends on the substratum: contributions of substrate adhesively and surface charge. Dev Biol. 1998;203:412-423.
Yang HY, Charles RP, Hummler E, Baines DL, Isseroff RR. The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes. J Cell Sci. 2013;126:1942-1951.
Onuma EK, Hui SW. Electric field-directed cell shape changes, displacement, and cytoskeletal reorganization are calcium dependent. J Cell Biol. 1998;106:2067-2075.
Dutta D, Asmar A, Stacey M. Effects of nanosecond pulse electric fields on cellular elasticity. Micron. 2015;72:15-20.
Titushkin I, Cho M. Regulation of cell cytoskeleton and membrane mechanics by electric field: Role of linker proteins. Biophys J. 2009;96:717-728.
Golberg A, Khan S, Belov V, et al. Skin rejuvenation with non-invasive pulsed electric fields. Sci Rep. 2015;12:10187.
TEGO Science, http://www.tegoscience.com/kor/main.do. http://www.tegocell.com/eng/neo/neo01.asp
Vinckier A, Semenza G. Measuring elasticity of biological materials by atomic force microscopy. FEBS Lett. 1998;430:12-16.
Bonifacio A, Sergo V. Effects of sample orientation in Raman microspectroscopy of collagen fibers and their impact on the interpretation of the Amide III band. Vib Spectrosc. 2012;53:314-317.
Shuster S, Black MM, McVitie E. The influence of age and sex on skin thickness, skin collagen and density. Br J Dermatol. 1975;93:639-643.
Gullekson C, Lucas L, Hewitt K, Kreplak L. Surface-sensitive Raman spectroscopy of collagen I fibrils. Biophys J. 2011;100:837-1845.
Nguyen TT, Gobinet C, Feru J, Brassart-Pasco S, Manfait M, Piot O. Characterization of Type I and IV collagens by Raman microspectroscopy: Identification of spectral markers of the dermo-epidermal junction. Spectroscopy. 2012;27:421-427.
Marek J, Polina D, Michael B, Albert Z, Robert WS. Anisotropic Raman scattering in collagen bundles. Opt Lett. 2010;35:2765-2767.
Alois B, Valter S. Effects of sample orientation in Raman microspectroscopy of collagen fibers and their impact on the interpretation of the amide III band. Vib Spectrosc. 2010;53:314-317.
Fraser RD, Macrae TP, Suzuki E. Chain conformation in the collagen molecule. J Mol Biol. 1979;129:463-481.
Rich A, Crick FHC. The structure of collagen. Nature. 1955;176:915-916.
Frushour BG, Koenig JL. Raman scattering of collagen, gelatin, and elastin. Biopolymers. 1975;14:379-391.
Jastrzebska M, Wrzalik R, Kocot A, Zalewska-Rejdak J, Cwalina B. Raman spectroscopic study of glutaraldehyde-stabilized collagen and pericardium tissue. J Biomater Sci Polym Ed. 2003;14:185-197.
Scoggin CH. The cellular basis of aging. West J Med. 1981;135:521-525.
McHugh D, Gil J. Senescence and aging: causes, consequences, and therapeutic avenues. J Cell Biol. 2018;217:65-77.
Gunin AG, Petrov VV, Vasilieva OV, Golubtsova NN. Age-related changes of blood vessels in the human dermis. Adv Gerontol. 2015;5:65-71.
Kim J, Cho SY, Kim SH, et al. Effects of Korean ginseng berry on skin antipigmentation and antiaging via FoxO3a activation. J Ginseng Res. 2017;41:277-283.
Sator PG. Skin treatments and dermatological procedures to promote youthful skin. Clin Interv Aging. 2006;1:51-56.
Diridollou S, Vabre V, Berson M, et al. Skin ageing: changes of physical properties of human skin in vivo. Int J Cosmet Sci. 2001;23:353-362.
Lo CM, Wang HB, Dembo M, Wang YL. Cell movement is guided by the rigidity of the substrate. Biophys J. 2000;79:4-152.
Kubitschke H, Schnauss J, Nnetu KD, Warmt E, Stange R, Kaes J. Actin and microtubule networks contribute differently to cell response for small and large strains. New J Phys. 2017;19:093003.
Lowery J, Kuczmarski ER, Herrmann H, Goldman RD. Intermediate filaments play a pivotal role in regulating cell architecture and function. Cell Mol Biol. 2015;290:17145-17153.
Brundage RA, Fogarty KE, Tuft RA, Fay FS. Calcium gradients underlying polarization and chemotaxis of eosinophils. Science. 1991;254:703-706.
Scherberich A, Campos-Toimil M, Ronde P, Takeda K, Beretz A. Migration of human vascular smooth muscle cells involves serum-dependent repeated cytosolic calcium transients. J Cell Sci. 2000;113:653-662.