Evaluation of presumptive identification of Enterobacterales using CHROMagar Orientation medium and rapid biochemical tests.
Enterobacterales
CHROMagar Orientation medium
presumptive identification
rapid biochemical tests
Journal
Journal of clinical laboratory analysis
ISSN: 1098-2825
Titre abrégé: J Clin Lab Anal
Pays: United States
ID NLM: 8801384
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
03
04
2020
revised:
07
05
2020
accepted:
29
05
2020
pubmed:
1
7
2020
medline:
14
8
2021
entrez:
29
6
2020
Statut:
ppublish
Résumé
The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is gradually spreading among large-scale laboratories; however, this method is impractical for small-scale laboratories. In laboratories without access to these rapid identification methods, problems related to them remain unsolved. In this study, we aimed to develop a rapid and inexpensive method to presumptively identify Enterobacterales using CHROMagar Orientation medium. The algorithm for presumptive identification of Enterobacteriaceae using CHROMagar Orientation medium was based on our previous studies. Modified property tests for indole, lysine decarboxylase, ornithine decarboxylase, and hydrogen sulfide were performed to evaluate the differentiation of the bacterial species. Using the type strains and clinical isolates, it was possible to conduct the property tests at a low cost, within 4 hours. The spot indole test was performed without any nonspecific reactions for the bacteria forming colored colonies. The presumptive identification of bacteria was thereby possible within 24 hours after specimen submission. All these results suggest that the rapid presumptive identification of Enterobacterales is possible with this new identification method using CHROMagar Orientation medium. This is therefore a prompt and economical method that can be used in routine laboratory work.
Sections du résumé
BACKGROUND
BACKGROUND
The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is gradually spreading among large-scale laboratories; however, this method is impractical for small-scale laboratories. In laboratories without access to these rapid identification methods, problems related to them remain unsolved. In this study, we aimed to develop a rapid and inexpensive method to presumptively identify Enterobacterales using CHROMagar Orientation medium.
METHODS
METHODS
The algorithm for presumptive identification of Enterobacteriaceae using CHROMagar Orientation medium was based on our previous studies. Modified property tests for indole, lysine decarboxylase, ornithine decarboxylase, and hydrogen sulfide were performed to evaluate the differentiation of the bacterial species.
RESULTS
RESULTS
Using the type strains and clinical isolates, it was possible to conduct the property tests at a low cost, within 4 hours. The spot indole test was performed without any nonspecific reactions for the bacteria forming colored colonies. The presumptive identification of bacteria was thereby possible within 24 hours after specimen submission.
CONCLUSION
CONCLUSIONS
All these results suggest that the rapid presumptive identification of Enterobacterales is possible with this new identification method using CHROMagar Orientation medium. This is therefore a prompt and economical method that can be used in routine laboratory work.
Identifiants
pubmed: 32594571
doi: 10.1002/jcla.23453
pmc: PMC7595914
doi:
Substances chimiques
Culture Media
0
Indoles
0
Carboxy-Lyases
EC 4.1.1.-
Ornithine Decarboxylase
EC 4.1.1.17
lysine decarboxylase
EC 4.1.1.18
Hydrogen Sulfide
YY9FVM7NSN
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23453Informations de copyright
© 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.
Références
J Clin Microbiol. 2013 Apr;51(4):1179-83
pubmed: 23363839
Am J Clin Pathol. 2012 Apr;137(4):620-6
pubmed: 22431539
J Clin Microbiol. 2000 Dec;38(12):4586-92
pubmed: 11101600
J Clin Lab Anal. 2020 Oct;34(10):e23453
pubmed: 32594571
Biochim Biophys Acta. 2015 Jun;1854(6):528-37
pubmed: 25448014
J Clin Microbiol. 2012 Oct;50(10):3301-8
pubmed: 22855510
Diagn Microbiol Infect Dis. 2017 Mar;87(3):207-209
pubmed: 27916545
J Infect Chemother. 2017 Apr;23(4):224-229
pubmed: 28161293
J Infect Chemother. 2018 Sep;24(9):769-772
pubmed: 29656900
J Clin Microbiol. 2004 Jan;42(1):60-4
pubmed: 14715732
Clin Microbiol Rev. 2016 Dec 14;30(1):381-407
pubmed: 27974411