Evidence for host-dependent RNA editing in the transcriptome of SARS-CoV-2.
APOBEC Deaminases
/ genetics
Adenosine Deaminase
/ genetics
Base Sequence
/ genetics
Betacoronavirus
/ genetics
Bronchoalveolar Lavage Fluid
/ virology
COVID-19
Coronavirus Infections
/ genetics
Genome, Viral
/ genetics
Host-Pathogen Interactions
/ genetics
Humans
Mutation Rate
Nucleotides
/ genetics
Pandemics
Pneumonia, Viral
/ genetics
RNA Editing
/ genetics
RNA, Viral
/ genetics
SARS-CoV-2
Transcriptome
Virus Replication
/ genetics
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
04
03
2020
accepted:
05
05
2020
entrez:
30
6
2020
pubmed:
1
7
2020
medline:
16
7
2020
Statut:
epublish
Résumé
The COVID-19 outbreak has become a global health risk, and understanding the response of the host to the SARS-CoV-2 virus will help to combat the disease. RNA editing by host deaminases is an innate restriction process to counter virus infection, but it is not yet known whether this process operates against coronaviruses. Here, we analyze RNA sequences from bronchoalveolar lavage fluids obtained from coronavirus-infected patients. We identify nucleotide changes that may be signatures of RNA editing: adenosine-to-inosine changes from ADAR deaminases and cytosine-to-uracil changes from APOBEC deaminases. Mutational analysis of genomes from different strains of Coronaviridae from human hosts reveals mutational patterns consistent with those observed in the transcriptomic data. However, the reduced ADAR signature in these data raises the possibility that ADARs might be more effective than APOBECs in restricting viral propagation. Our results thus suggest that both APOBECs and ADARs are involved in coronavirus genome editing, a process that may shape the fate of both virus and patient.
Identifiants
pubmed: 32596474
doi: 10.1126/sciadv.abb5813
pii: abb5813
pmc: PMC7299625
doi:
Substances chimiques
Nucleotides
0
RNA, Viral
0
ADA protein, human
EC 3.5.4.4
Adenosine Deaminase
EC 3.5.4.4
APOBEC Deaminases
EC 3.5.4.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
eabb5813Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
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