Genome-Wide DNA Methylation Patterns in Children Exposed to Nonpharmacologically Treated Prenatal Depressive Symptoms: Results From 2 Independent Cohorts.
ALSPAC
ARIES
DNA methylation
FRANCES
Pregnancy
epigenetics
prenatal depression
Journal
Epigenetics insights
ISSN: 2516-8657
Titre abrégé: Epigenet Insights
Pays: United States
ID NLM: 101735398
Informations de publication
Date de publication:
2020
2020
Historique:
received:
10
02
2020
accepted:
01
05
2020
entrez:
30
6
2020
pubmed:
1
7
2020
medline:
1
7
2020
Statut:
epublish
Résumé
Maternal depressive symptoms are a common phenomenon during pregnancy and are related to negative outcomes for child development and health. Modifications in child DNA methylation are discussed as an underlying mechanism for the association between prenatal depressive symptoms and alterations in child outcomes. However, formerly reported genome-wide associations have yet to be replicated. In an epigenome-wide association study (EWAS), alterations of DNA methylation related to maternal prenatal depressive symptoms were investigated in buccal cell samples from 174 children (n = 52 exposed to prenatal depressive symptoms; 6-9 years old) of the German longitudinal study FRAMES-FRANCES. Whole blood samples from the independent, age-comparable ARIES subsample of the ARIES/ALSPAC study (n = 641; n = 159 exposed to prenatal depressive symptoms; 7-8 years old) were examined as a confirmation sample. Depressive symptoms were assessed with the Edinburgh Postnatal Depression Scale. DNA methylation was analyzed with the Infinium Human Methylation 450k BeadChip. Modifications in single CpGs, regions, and biological pathways were investigated. Results were adjusted for age and birth outcomes as well as postnatal and current maternal depressive symptoms. Analyses were performed for the whole sample as well as separated for sex. The EWAS yielded no differentially methylated CpG or region as well as no accordance between samples withstanding correction for multiple testing. In pathway analyses, no overlapping functional domain was found to be enriched for either sample. A comparison of current and former findings suggests some overlapping methylation modifications from infancy to childhood. Results suggest that there might be sex-specific differential methylation, which should be further investigated in additional studies. The current, mainly nonsignificant, results challenge the assumption of consistent modifications of DNA methylation in children exposed to prenatal depressive symptoms. Despite the relatively small sample size used in this study, this lack of significant results may reflect diverse issues of environmental epigenetic studies, which need to be addressed in future research.
Sections du résumé
BACKGROUND
BACKGROUND
Maternal depressive symptoms are a common phenomenon during pregnancy and are related to negative outcomes for child development and health. Modifications in child DNA methylation are discussed as an underlying mechanism for the association between prenatal depressive symptoms and alterations in child outcomes. However, formerly reported genome-wide associations have yet to be replicated.
METHODS
METHODS
In an epigenome-wide association study (EWAS), alterations of DNA methylation related to maternal prenatal depressive symptoms were investigated in buccal cell samples from 174 children (n = 52 exposed to prenatal depressive symptoms; 6-9 years old) of the German longitudinal study FRAMES-FRANCES. Whole blood samples from the independent, age-comparable ARIES subsample of the ARIES/ALSPAC study (n = 641; n = 159 exposed to prenatal depressive symptoms; 7-8 years old) were examined as a confirmation sample. Depressive symptoms were assessed with the Edinburgh Postnatal Depression Scale. DNA methylation was analyzed with the Infinium Human Methylation 450k BeadChip. Modifications in single CpGs, regions, and biological pathways were investigated. Results were adjusted for age and birth outcomes as well as postnatal and current maternal depressive symptoms. Analyses were performed for the whole sample as well as separated for sex.
RESULTS
RESULTS
The EWAS yielded no differentially methylated CpG or region as well as no accordance between samples withstanding correction for multiple testing. In pathway analyses, no overlapping functional domain was found to be enriched for either sample. A comparison of current and former findings suggests some overlapping methylation modifications from infancy to childhood. Results suggest that there might be sex-specific differential methylation, which should be further investigated in additional studies.
CONCLUSIONS
CONCLUSIONS
The current, mainly nonsignificant, results challenge the assumption of consistent modifications of DNA methylation in children exposed to prenatal depressive symptoms. Despite the relatively small sample size used in this study, this lack of significant results may reflect diverse issues of environmental epigenetic studies, which need to be addressed in future research.
Identifiants
pubmed: 32596638
doi: 10.1177/2516865720932146
pii: 10.1177_2516865720932146
pmc: PMC7298426
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2516865720932146Subventions
Organisme : Medical Research Council
ID : MC_PC_19009
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_15018
Pays : United Kingdom
Organisme : NICHD NIH HHS
ID : R01 HD068437
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI121226
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Declaration of Conflicting Interests:The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Hartmut Heinrich works at the neuroCare Group, Munich, and is Research Fellow at the Research Institute Brainclinics, Nijmegen.
Références
Epigenetics. 2014 Jul;9(7):964-72
pubmed: 24751725
Mol Psychiatry. 2016 Mar;21(3):339-47
pubmed: 26008736
Int J Epidemiol. 2015 Aug;44(4):1181-90
pubmed: 25991711
Epigenetics. 2008 Mar-Apr;3(2):97-106
pubmed: 18536531
Nat Genet. 2013 Dec;45(12):1452-8
pubmed: 24162737
Nat Rev Genet. 2009 May;10(5):295-304
pubmed: 19308066
Acta Physiol (Oxf). 2011 Jun;202(2):103-18
pubmed: 21401888
PLoS One. 2013 May 17;8(5):e63812
pubmed: 23691101
BMC Bioinformatics. 2012 May 08;13:86
pubmed: 22568884
Bioinformatics. 2016 Sep 1;32(17):2604-10
pubmed: 27187204
Alcohol. 2016 Aug;54:39-44
pubmed: 27565755
Epigenetics. 2012 Jul;7(7):735-46
pubmed: 22677950
Neuroscience. 2017 Feb 7;342:154-166
pubmed: 26343292
Epigenetics. 2015;10(5):408-17
pubmed: 25875334
Nat Genet. 2019 Mar;51(3):431-444
pubmed: 30804558
Nat Protoc. 2009;4(1):44-57
pubmed: 19131956
J Dev Orig Health Dis. 2017 Apr;8(2):244-255
pubmed: 28103963
Sci Rep. 2018 May 2;8(1):6944
pubmed: 29720614
Annu Rev Clin Psychol. 2019 May 7;15:317-344
pubmed: 30795695
Nord J Psychiatry. 2019 May - Jul;73(4-5):257-263
pubmed: 31070508
Nat Rev Mol Cell Biol. 2019 Oct;20(10):590-607
pubmed: 31399642
Environ Health Perspect. 2017 Apr;125(4):511-526
pubmed: 28362264
Fortschr Neurol Psychiatr. 2009 Dec;77(12):708-13
pubmed: 19859869
PLoS One. 2012;7(7):e41361
pubmed: 22848472
Int J Epidemiol. 2018 Feb 1;47(1):22-23u
pubmed: 29025028
Transl Psychiatry. 2016 Sep 13;6(9):e890
pubmed: 27622934
Curr Psychiatry Rep. 2015 Feb;17(2):5
pubmed: 25617041
Nat Commun. 2019 Jun 11;10(1):2548
pubmed: 31186427
Sci Rep. 2019 Apr 18;9(1):983
pubmed: 31000782
Psychoneuroendocrinology. 2017 Oct;84:101-108
pubmed: 28697416
Dev Psychopathol. 2019 May;31(2):419-431
pubmed: 29606180
Int J Epidemiol. 2013 Feb;42(1):111-27
pubmed: 22507743
Transl Psychiatry. 2018 Dec 3;8(1):266
pubmed: 30510187
Environ Res. 2016 Jan;144(Pt A):139-148
pubmed: 26610292
Am J Psychiatry. 2017 Apr 1;174(4):319-328
pubmed: 27838934
Stress. 2011 Jul;14(4):348-56
pubmed: 21663536
Transl Psychiatry. 2018 Nov 7;8(1):244
pubmed: 30405117
Epigenetics. 2012 Jun 1;7(6):594-605
pubmed: 22522910
Sci Rep. 2014 Jan 22;4:3807
pubmed: 24448323
Neuropsychopharmacology. 2009 May;34(6):1363-80
pubmed: 18536703
Child Dev. 2013 Jan-Feb;84(1):49-57
pubmed: 22880724
Transl Psychiatry. 2015 Apr 07;5:e545
pubmed: 25849984
Annu Rev Clin Psychol. 2008;4:275-303
pubmed: 18370618
Physiol Rev. 2014 Oct;94(4):1027-76
pubmed: 25287859
Cancer Manag Res. 2018 Nov 26;10:6339-6355
pubmed: 30568496
Physiol Behav. 2012 Jul 16;106(5):736-40
pubmed: 22353310
Int J Epidemiol. 2013 Feb;42(1):97-110
pubmed: 22507742
J Am Acad Child Adolesc Psychiatry. 2001 Nov;40(11):1337-45
pubmed: 11699809
Cells. 2019 Aug 21;8(9):
pubmed: 31438539
J Neurosci. 2008 Sep 3;28(36):9055-65
pubmed: 18768700
J Child Psychol Psychiatry. 2011 Apr;52(4):442-75
pubmed: 21309772
Genomics. 2011 Oct;98(4):288-95
pubmed: 21839163
Chem Res Toxicol. 2017 Oct 16;30(10):1911-1920
pubmed: 28927277
J Child Psychol Psychiatry. 2018 Apr;59(4):303-322
pubmed: 28736860
Bioinformatics. 2018 Dec 1;34(23):3983-3989
pubmed: 29931280
Front Behav Neurosci. 2016 Jul 12;10:147
pubmed: 27462209
Mol Psychiatry. 2017 Feb;22(2):250-256
pubmed: 27217153
Transl Psychiatry. 2016 Sep 20;6(9):e895
pubmed: 27648917
J Affect Disord. 2015 May 1;176:95-105
pubmed: 25704562
Methods Mol Biol. 2010;620:243-65
pubmed: 20652507
Mol Psychiatry. 2014 Oct;19(10):1071-7
pubmed: 25199917
Genome Res. 2012 Apr;22(4):623-32
pubmed: 22300631
Nucleic Acids Res. 2009 Jan;37(1):1-13
pubmed: 19033363
Epigenetics. 2019 Feb;14(2):109-117
pubmed: 30821575
Epigenomics. 2017 Mar;9(3):313-332
pubmed: 28140666
Br J Psychiatry. 1987 Jun;150:782-6
pubmed: 3651732
J Transl Med. 2019 Jan 9;17(1):15
pubmed: 30626398
J Am Acad Child Adolesc Psychiatry. 2018 May;57(5):321-328.e2
pubmed: 29706161
Infant Ment Health J. 2019 Jul;40(4):513-522
pubmed: 31066465
Epigenetics. 2012 Apr;7(4):409-14
pubmed: 22419064
Bioinformatics. 2011 Jun 1;27(11):1496-505
pubmed: 21471010
Epigenetics Chromatin. 2013 Mar 03;6(1):4
pubmed: 23452981
Genes (Basel). 2016 Dec 03;7(12):
pubmed: 27918480
Genome Biol. 2014 Dec 03;15(12):503
pubmed: 25599564
Infant Ment Health J. 2017 Jan;38(1):15-52
pubmed: 28042663
Cell Rep. 2018 Jun 26;23(13):3864-3877
pubmed: 29949770
Z Kinder Jugendpsychiatr Psychother. 2018 Mar;46(2):155-167
pubmed: 28256157
Matern Child Health J. 2015 Jun;19(6):1306-37
pubmed: 25452215
Dev Psychopathol. 2018 Aug;30(3):807-824
pubmed: 30068415
Genome Biol. 2013;14(10):R115
pubmed: 24138928
PLoS One. 2016 Jul 18;11(7):e0159093
pubmed: 27427907
PLoS One. 2015 Aug 28;10(8):e0135849
pubmed: 26317609
Am J Psychiatry. 2016 Jul 1;173(7):705-13
pubmed: 27013342
Stress. 2011 Nov;14(6):581-9
pubmed: 21854166
Epigenetics. 2013 Dec;8(12):1321-9
pubmed: 24135662
Nutrients. 2018 Apr 06;10(4):
pubmed: 29642382
Nucleic Acids Res. 2018 Jul 6;46(12):5977-5995
pubmed: 29722878