Temporal and Concentration Effects of Methanol on Cryopreservation of Zebrafish (

Animals Cryopreservation / methods Cryoprotective Agents / pharmacology Male Methanol / pharmacology Semen Preservation / methods Spermatozoa / physiology Zebrafish / physiology
cryoprotectant fertilization motility plasma membrane integrity sperm cell survival

Journal

Zebrafish
ISSN: 1557-8542
Titre abrégé: Zebrafish
Pays: United States
ID NLM: 101225070

Informations de publication

Date de publication:
08 2020
Historique:
pubmed: 1 7 2020
medline: 20 3 2021
entrez: 30 6 2020
Statut: ppublish

Résumé

We evaluated the cryoprotective effects of methanol on zebrafish sperm at different concentrations, exposure times, and stages during cryopreservation. Samples were collected by crushing of dissected testes or abdominal stripping. After exposure to 0%, 2%, 5%, 8%, and 10% methanol for 0-11 min, fresh sperm (1 × 10

Identifiants

DOI: 10.1089/zeb.2019.1849 PMID: 32598236 PMC: PMC7455470
pubmed: 32598236
doi: 10.1089/zeb.2019.1849
pmc: PMC7455470
doi:

Substances chimiques

Cryoprotective Agents 0
Methanol Y4S76JWI15

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

233-242

Subventions

Organisme : NIH HHS
ID : P40 OD011021
Pays : United States
Organisme : NIH HHS
ID : R24 OD010441
Pays : United States
Organisme : NIH HHS
ID : R24 OD011120
Pays : United States

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Auteurs

Huiping Yang (H)

School of Forest Resources and Conservation, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida, USA.
Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA.

E Hu (E)

Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA.
Center for Aquaculture Technologies, Inc., San Diego, California, USA.

Terrence Tiersch (T)

Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA.

Carrie Carmichael (C)

Zebrafish International Resource Center, 5274 University of Oregon, Eugene, Oregon, USA.

Jen Matthews (J)

Zebrafish International Resource Center, 5274 University of Oregon, Eugene, Oregon, USA.

Zoltan M Varga (ZM)

Zebrafish International Resource Center, 5274 University of Oregon, Eugene, Oregon, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Temporal and Concentration Effects of Methanol...
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Cryoconservation › Temporal and Concentration Effects of Methanol...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Cryoprotecteurs › Temporal and Concentration Effects of Methanol...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Méthanol › Temporal and Concentration Effects of Methanol...
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Conservation de tissu › Conservation de semence › Temporal and Concentration Effects of Methanol...
questionsmedicales.fr › Cellules › Cellules germinales › Spermatozoïdes › Temporal and Concentration Effects of Methanol...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › Temporal and Concentration Effects of Methanol...