Detection of circulating cell-free DNA to diagnose Schistosoma japonicum infection.
Animals
Biomarkers
/ blood
Cell-Free Nucleic Acids
/ blood
Cercaria
/ isolation & purification
DNA, Intergenic
/ blood
Humans
Male
Mice
Mice, Inbred BALB C
Molecular Diagnostic Techniques
Rabbits
Schistosoma japonicum
/ genetics
Schistosomiasis
/ diagnosis
Schistosomiasis japonica
/ genetics
Serum
/ parasitology
Snails
Cell-free DNA
Internal transcribed spacer 2
Molecular diagnosis
Schistosoma japonicum
Journal
Acta tropica
ISSN: 1873-6254
Titre abrégé: Acta Trop
Pays: Netherlands
ID NLM: 0370374
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
16
12
2019
revised:
17
06
2020
accepted:
25
06
2020
pubmed:
1
7
2020
medline:
5
1
2021
entrez:
30
6
2020
Statut:
ppublish
Résumé
Schistosomiasis occurs in 240 million people worldwide and is a major public health concern. Thus, early diagnosis and monitoring of schistosomiasis progression are needed to treat patients. Cell-free DNA (cfDNA) is present as fragments of parasite-derived DNA in host body fluids. Detection of this cfDNA in host blood may be a promising diagnostic marker of schistosomiasis. Therefore, in this study, we investigated the potential of internal transcribed spacer 2 (ITS2), a molecular taxonomy and barcoding marker, in diagnosing schistosomiasis using infected rabbit and mice sera. A 192 bp fragment of ITS2 was detected in the serum-isolated DNA from the infected host on different days after infection. We also determined the sensitivity of detecting ITS2 in mice with varying numbers of cercaria: cfDNA was present even in mice with low abundance of the parasite. Overall, our results show that cfDNA may be a potential tool for the early diagnosis and therapeutic evaluation of S. japonicum infection.
Identifiants
pubmed: 32598919
pii: S0001-706X(19)31767-X
doi: 10.1016/j.actatropica.2020.105604
pii:
doi:
Substances chimiques
Biomarkers
0
Cell-Free Nucleic Acids
0
DNA, Intergenic
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
105604Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that there are no conflicts of interest.